Describe the following techniques for determining gene or protein function with advantages disadvantages and limitations. i)forward genetics(random mutagenesis) ii)reverse genetics(altering the function of particular gene) iii)genetic analysis(double or higher order mutant combination)

Select all that apply: A SNP (single nucleotide polymorphism) is similar to an STR (short tandem repeat) in that: a) an individual can have many different copies of specific SNP or STR b) they both cause diseases c)they are sequences that have many possible variants d) an individual has one copy of this region of the genome from each parent.

Three mutations were obtained in a bacterial gene. An antibody is available for the protein product of this gene. Both Northern analysis (RNA separated by electrophoresis, blotted, and probed with DNA) and Western analysis (proteins separated by electrophoresis, blotted, and probed with antibodies) were performed on the mutants. The results are summarized below. Northern Size Western Size 1 2 3 1 2 3 + Long Short Short Long For each mutation, what kind of mutation occurred and how do you know? a) Mutant 1 b) Mutant 2 c) Mutant 3

Which of the following conditions is most likely to be successfully treated by gene therapy using a viral vector to deliver a wild-type copy of one gene that is present in mutant form in a person with the condition? Assume that the viral vector used has the ability to home to relevant target tissues. Also assume that the patient can be treated at a young enough age to avoid irreversible phenotypic impacts of the mutation described. Timothy Syndrome, a multi-system disorder characterized by dysmorphic features and autistic behavioral traits, caused by overexpression of calcium channel gene Cerebral adrenoleukodystrophy (ALD), a neurological disorder caused by a frameshift mutation knocking out the function of the ABCD1 gene encoding a lipid transporter Osteogenesis imperfecta caused by a dominant negative mutation in the collagen A1 gene Myopia (shortsightedness), a vision impairment with heritability estimates in the range of 0.6-0.8, where risk is impacted by at least 200 genes