Explain the Detection of the sickle-cell mutation by sequencing of PCR products?

Q: Why is PCR needed for SNP analysis?

A: PCR stands for the polymerase chain reaction discovered by Kary Mullis, which was the mechanism used…

Q: In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a)…

A: Polymerase chain reaction or PCR is a technique of formation of millions to billions of copies of a…

Q: What is the possible cause of the presence of an extra band in the results of DNA extraction and PCR…

A: This finding suggests that formation of multiple bands in non-denaturing gel electrophoresis is a…

Q: What is real-time PCR?

A: Besides tools, there a several techniques which are used in recombinant DNA technology. These…

Q: Why might exome sequencing fail to identify a diseasecausing mutation in an affected person?

A: Exome sequencing can only detect variants in the coding region of genes that have an impact on…

Q: Why Gene-prediction programs are used ?

A: In a DNA sequence, where those codons might fall even where a functional protein might be present or…

Q: what are the variants of PCR ?

A: PCR ( polymerase chain reaction) is a test tube method for amplifying a selected DNA sequence. There…

Q: Why is PCR beneficial?

A: PCR is a very useful tool and has many applications in various fields such as medical, forensic,…

Q: Who proposed shotgun sequencing?

A: The method used for the sequencing of random DNA strands is genetics, shotgun sequencing.  This is…

Q: What detection method could be used to determine the mutagen added to a microbial culture resulting…

A: The mutagens are chemical mixtures or a type of radiation that cause irreversible and heritable…

Q: In microbial genetics, what is referred to as Griffith effect?

A: Microbial genetics is a subject area within microbiology and genetic engineering. Microbial genetics…

Q: Describe how Ames Test works to identify carcinogenic using back mutations (reversible)

A: The Ames test is a widely employed method that uses bacteria to test whether a given chemical can…

Q: After four cycles of PCR, which products predominate? Explain why.

A: Polymerase chain reaction (PCR) is a type of genetic analysis in which the nucleotide sequence of…

Q: How did the private corporation Celera Genomics approach the sequencing of the human genome? What…

A: Celera Genomics is a private biotechnology company that raced with the IHGSC to complete the…

Q: why are DTT and Mg added to PCRs? what is their affect and role in there?

A: The process of obtaining multiple copies of a known DNA sequence is known as gene amplification. It…

Q: Explain the Detection of simple sequence repeat (SSR) polymorphisms by electrophoresis of PCR…

A: Polymerase Chain Reaction is a technique to generate the copies or to amplify the DNA region…

Q: Why is extra primers added to the pcr reaction?

A: Introduction :- The polymerase chain reaction, or PCR, is a chemical reaction used by molecular…

Q: Using the question in the image below, identify the locations of each mutation in strains 1, 2, and…

A: Genes are short segments of DNA. They are responsible for our attributes, which are things like hair…

Q: Discuss why a genetic disease might have a particular age of onset.Would an infectious disease have…

A: Different genetic diseases result in different abnormalities in the human genome. A genetic disorder…

Q: Name four reagents used during a PCR reaction

A: Polymerase chain reaction: It is an in vitro technique that results in the exponential…

Q: What do you think will happen if the melting temperatures of the PCR primers (forward and reverse)…

A: PCR (Polymerase Chain Reaction) is a technique, which is used to amplify a small fragment of DNA and…

Q: Why Touch-down PCR technique is better than the Gradient PCR technique? Also, from those techniques…

A: The PCR or polymerase chain reaction is a technique to enhance or amplify DNA sequences in vitro by…

Q: Explain about Multiple PCR cycles ?

A: PCR stands for a polymerase chain reaction in which multiple copies of DNA molecules can be obtained…

Q: Explain The Discovery of CRISPR ?

A: The common technology   that can be used to edit genes is refers as the CRISPR. In other words you…

Q: Will any of the PCR reaction components (e.g., dNTPs, primer) become limiting before the product is…

A: Polymerase Chain Reaction (PCR): It is a molecular technique for making multiple copies of DNA…

Q: It is possible to diagnose genetic mutation by PCR. Explain

A: The process of finding unknown or known mutations in the genes or genetic material is known as…

Q: What is transgenic mutation?

A: Mutation is the sudden heritable changes that occur in the DNA sequences due to error while copying…

Q: What are some possible reasons that researchers might be interested in identifying the gene that…

A: ICA(Internal Carotid Artery) is a part of the vascular system which is susceptible to Carotid artery…

Q: Explain why PCR results in the amplification of a specific DNA sequence despite many competing…

A: PCR is a very powerful and practical research tool which uses sequencing and identification DNA…

Q: How to find unknown gene and design primers present using whole genome sequence?

A: Step 1 Whole genome sequencing is the process that regulates the complete deoxyribonucleic acid…

Q: Briefly answer the question: Exome sequencing to identify a mutation that could cause a particular…

A: Primary findings are the testing results which are obtained and provide required information about…

Q: Discuss the role (presence and absence) of a selectable marker (selection) in cloning a recombinant…

A: Recombinant DNA molecule is defined the molecule in which an isolated DNA fragment or gene of…

Q: How is a genome-wide association study carried out?

A: Deoxyribonucleic acid (DNA) is a genetic material of most organisms. DNA contains the instructions…

Q: Why PCR Products Are Genotypedby Sequencing or SizingFor Mendelian genetic diseases cau?

A: Karry Mullis invented this ingenious method in 1989, who was awarded Nobel prize in 1993. Polymerase…

Q: What are the major considerations in the design of PCR cloning primers?

A: INTRODUCTION Primers It is a short nucleic acid sequence that act as a starting point of DNA…

Q: Explain why PCR generates billions of products that are all the same size

A: PCR is Polymerase Chain Reaction, is highly used for the production or amplification of original DNA…

Q: Explain the possible repercussions to an organism, when a mutation occurs in the genome?

A: Mutation   The change or variation occurs in the sequence of DNA is known as mutation. In mutation…

Q: What is a transgenic organism? Describe three examples.

A: In living organisms, genome can be altered or modified with the technology known as genetic…

Q: Why do you detect mutations one at a time on PCR? Is there any way to detect multiple mutations in…

A: PCR that is Ploymerase chain reaction is a molecular technique used in the laboratories to amplify…

Q: What are five variables to consider when designing a primer for standard PCR and state why.

A: Polymerase chain reaction involves amplification of desired DNA strand using three steps i.e..…

Q: Explain the rationale behind the use of the “bag of worms” phenotype as a way to identify…

A: A mutation refers to an alteration in the genome or genetic material of a living organism’s cells or…

Q: What is the Ames test and how is it carried out? What assumption concerning mutagenicity and…

A: Mutagens are chemical compounds that cause changes in the DNA. These changes are irreversible and…

Q: Describe the three steps of a PCR cycle.

A: Polymerase chain reaction is a molecular biology technique used to amplify a single copy of DNA that…

Q: For each of the following experimental goals, is PCR orgene cloning preferable and why?a. Isolate…

A: Introduction The polymerase chain reaction (PCR) is a widely used method for rapidly making millions…

Q: Why is it practical to test carcinogens by a mutagenesis assay?

A: Carcinogens are the cancer causing substances. Cancer is an uncontrolled division of cell that has a…

Q: Explain the example of predominant method for sequencing genomes ?

A: The study of genomes' design, functioning, development, sequencing, and modification is the emphasis…

Hematology

Hematology is the branch of biology. Hematology is the medical field that deals with the treatment of blood disorders and cancers, such as hemophilia, leukemia, lymphoma, and sickle-cell anemia. Hematologists and hematopathologists are medical professionals who specialize in illnesses of the blood and its components.