Describe the application of and theory behind PR broth, including how the various forms of this test relate to organism metabolism.

Briefly describe specifically how the presence of our enzyme of interest is being detected/observed in Urease test.

Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. 1.You intend to add 9ml of broth (water) to each tube and 1 ml of culture. Instead, you add 5 ml of broth to each tube and 5 ml of culture to the first tube. Then, you make a serial dilution of 1 ml into and from each tube as described. 2. You prepare 9 ml of broth (water) in each tube. You add 1ml of culture of every tube. 3.You add 9 ml of broth (water) to each tube. You add 1 ml of culture to the first tube and mix. You get distracted, and transfer 1ml of the dilutant (mixture of culture and broth) to the third tube instead of the second. You perform the rest of the series as described.

Sugar Tube results are reliable if judged at 24-48 hours. I set up my Glucose Sugar Tube on Tuesday. It starts out all red, no gas. On Thursday I get the result shown at the left, Acid Production and no gas. But the next Tuesday it is all red with no gas. What happened? Remember that the media has both one sugar and beef extract.