Describe briefly Immunofixation Technique

what principles are used for administering immunotherapeutic agents?

In which type of ELISA antigen exist in between the two antibodies?     Direct     Indirect     Sandwich     Competitive

Describe the background aims of immunologic testing.

The direct ELISA test requires     only one antibody     only one antigen     both primary and secondary antibody     only secondary antibody conjugated with enzyme system

Explain why IgM has a stronger power of agglutination than antibodies of any other class.

How are antivenomsproduced? Why areantivenoms an example ofpassive immunization?

1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge  true/false 3)  PBST  is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme  A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8

Describe 2 methods of immunotherapy currently in use, and the clinical results of one of these (ie, how often does it work well?)

Interpret the outcome of direct and indirect immunofluorescentantibody testing.