CRISPR/Cas9 gene editing tool is adapted from the _ immune systems.
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- What does the acronym CRISPR stand for? Briefly outline the components of the CRISPR/Cas system. What is the function of the CRISPR/Cas system?What do you think about the ethical impacts of a technology such as CRISPR? What is the main parameter that is used to define new virus families among archaeal viruses? List and outline that various stages through which bacteria and archaea fight back against infection with viruses.Reverse transcriptase would most likely be found in which of the following types of viruses? an RNA-based lytic virus a DNA-based lytic virus an RNA-based lysogenic virus a DNA-based lysogenic virus CO000Please place the stages of a CRISPR-cas9 gene editing workflow in the correct order below. Note, there is one incorrect and state why? Sequence Select for correctly gene edited cells (using antibiotic resistance and/or colour production for example) Clonal isolation Synthesise DNA insert oligonucleotides Clonal characterisation (analysis of phenotype) Add green fluorescent protein gene sequence into plasmid to aid selection of correctly transfected cells. Transfect cells Clone into CRISPR-cas9 expression vector Purify plasmids Design a set of targeting sequences using algorithms.
- Remdesivir is an antiviral drug used to treat SARS-CoV-2 infections. It is a broad-spectrum antiviral, meaning it has activity against many different viruses. Remdesivir can be incorporated into a new RNA chain as a virus replicates and can interfere with the viral replication machinery. Template RNA Elongation New RNA SARS-CoV-2 RNA polymerase Template groove Nucleotide entrance Nucleotides Remdesivir Figure 1. A representation of remdesivir and the viral replication machinery for SARS-CoV-2. 6. Figure 1 shows that remdesivir "mimics” an important component of RNA replication. Which specific component of RNA replication has a structure like that of remdesivir? 7. Propose a hypothesis about how remdesivir might inhibit the virus's replication process.OF rasc PDF Teas fill Which of the following is NOT a step in the replication of a retrovirus? A diagram illustrating the process is provided below. PDF UNOFFIC TRANSCR 59°F Mostly cloudy Virus Viral RNA Reverse transcriptase DDDDDD ↓Viral DNA Provirus mRNA mm Proteins mn Viral RNA Protease Plasma membrane Host DNA New virus O The single-stranded DNA forms a double-stranded DNA called a provirus, which is incorporated into the host cell DNA. O After a retrovirus attaches to the host cell, it injects its viral DNA and uses the host cell's machinery and materials to replicate. O After a retrovirus injects its viral RNA into a host cell, it forms a DNA strand by reverse transcription. O When the host cell replicates, a provirus produces the viral RNA needed to produce more virus particles. P O Search hun recc PDF maste omissAlthough CRISPR uses some of the same viral vectors as "gene therapy," which was pioneered in the 1990s the cargo is more targeted. True False
- Please help explain this sentence Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen Alice Mac Kain 1,12, Ghizlane Maarifi2,12, Sophie-Marie Aicher3, Nathalie Arhel 2, Artem Baidaliuk 4, Sandie Munier5,6, Flora Donati 5,6, Thomas Vallet 1, Quang Dinh Tran 1, Alexandra Hardy 1, Maxime Chazal 3, Françoise Porrot 7, Molly OhAinle 8, Jared Carlson-Stevermer 9, Jennifer Oki9, Kevin Holden 9, Gert Zimmer 10, Etienne Simon-Lorière 4, Timothée Bruel 7, Olivier Schwartz 7, Sylvie van der 5,6, Nolwenn Jouvenet 3✉, Sébastien Nisole 2✉, Marco Vignuzzi 1✉ &Which of the following viral nucleic acids can be used as a template to produce mRNA? Select all that apply. plus strand DNA plus strand RNA minus strand RNA minus strand DNAWhich of the following is NOT true about transduction? The bacteriophage's own viral DNA is the source of new genes for bacterial recombination Pieces of donor bacterial DNA can be inserted into the recipient bacterial cell's chromosome None of the other four answers (All are true statements about transduction) O Genetic recombination can result from bacteriophage (phage) virus infection of bacteria During the assembly of new phage particles, pieces of bacterial DNA are sometimes mistakenly inserted into new virus particles instead of phage (viral) DNA
- What enzyme is required for the movement of transposable elements that involve an RNA intermediate in virus? options: RNA polymerase reverse transcriptase polyA polymerase peptidyl transferaseHow do some bacteria protect themselves against the viruses? protect themselves by making antibodies mutating the virus genome revers transcriptase assemble the capsid restriction enzymeWhich of the following are ways bacteria have of dealing with viral infection? RNA interference Raising body temperature Monoclonal antibodies Restriction enzymes CRISPR-Cas system