Consulting Table 4.1, determine the length and the nature (59 or 39) of the overhang (if any) created by the following restriction endonucleases: a. Alul b. BglII c. ClaI d. Kpnl e. Mbol f. Pxul g. NotI Table 4.1 Recognition Sequences and Cutting Sites of Selected Restriction Endonucleases Enzyme Recognition Sequence* Alul AGICT BamHI GIGATCC Bgll AIGATCT Clal ATICGAT EcoRI GIAATTC Haell GGICC Hindll GTPY I Pu AC Hindll AJAGCIT Нрall CICGG Kpnl GGTACIC Mbol ĮGATC Pstl CTGCALG CGATICG GITCGAC Pvul Sall Smal CCCIGGG Xmal CICCGGG Notl GCIGGCCGC

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
icon
Concept explainers
Question
100%
Consulting Table 4.1, determine the length and the nature (59 or 39) of the overhang (if any)
created by the following restriction endonucleases:
a. Alul
b. Bglll
с. Clal
d. Кpnl
e. Mbol
f. Pyul
g. Not!
Table 4.1 Recognition Sequences and Cutting
Sites of Selected Restriction
Endonucleases
Enzyme
Recognition Sequence*
Alul
AGICT
BamHI
GIGATCC
Bgl|
AIGATCT
Clal
ATICGAT
EcoRI
GIAATTC
Haelll
GGICC
GTPY I Pu A C
ALAGCIT
Hindll
Hindll
Нраll
CICGG
Kpnl
GGTACIC
Mbol
ĮGATC
Pstl
CTGCALG
Pvul
CGATICG
Sall
GITCGAC
Smal
CCCIGGG
Xmal
CICCGGG
Notl
GCIGGCCGC
*Only one DNA strand, written 5'3' left to right is presented, but restriction
endonucleases actually cut double-stranded DNA as illustrated in the text for EcoRI.
The cutting site for each enzyme is represented by an arrow.
Transcribed Image Text:Consulting Table 4.1, determine the length and the nature (59 or 39) of the overhang (if any) created by the following restriction endonucleases: a. Alul b. Bglll с. Clal d. Кpnl e. Mbol f. Pyul g. Not! Table 4.1 Recognition Sequences and Cutting Sites of Selected Restriction Endonucleases Enzyme Recognition Sequence* Alul AGICT BamHI GIGATCC Bgl| AIGATCT Clal ATICGAT EcoRI GIAATTC Haelll GGICC GTPY I Pu A C ALAGCIT Hindll Hindll Нраll CICGG Kpnl GGTACIC Mbol ĮGATC Pstl CTGCALG Pvul CGATICG Sall GITCGAC Smal CCCIGGG Xmal CICCGGG Notl GCIGGCCGC *Only one DNA strand, written 5'3' left to right is presented, but restriction endonucleases actually cut double-stranded DNA as illustrated in the text for EcoRI. The cutting site for each enzyme is represented by an arrow.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

Blurred answer
Knowledge Booster
DNA and RNA
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education