Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Question
Considering that 22.4 kJ/mole is required to transport 1 H+ across the mitochondrial membrane,
how much energy is available for proton translocation from the oxidation of FADH2 at Complex II?
Answer: 134 kJ/mol
Can you please explain why the answer would be 134 kJ/mol?
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- Aconitase catalyzes the conversion of Citrate to Isocitrate in the TCA cycle. The standard free energy change (AG°) for this reaction is +6.7 kJ/mol. The observed free energy change (AG) for the reaction in pig heart mitochondria is +0.8 kJ/mol. The ratio of [isocitrate]/[citrate] in these mitochondria is: Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a 10 b. 1 100 0.01 e f 0.33 0.10 h 0.25 i 2arrow_forwardYou are isolating mitochondria from insect cells and incubating in a test tube with 0.005 M FADH2, 0.05 M ADP and 0.05 M Pi. Assuming, these 3 can enter mitochondria at no cost,no glucose/products of glucose metabolism remain in the isolated mitochondria, and oxygen is present. Part 1) If all expected reactions go to completion, how much ATP is expected formed? (0 M)(0.02 M) (0.03 M) (0.05 M) (0.1 M) (0.005 M) (0.01 M) (0.015 M) Part 2) The ratio of FADH2/FAD at completion of all expected reactions would be? (2) (>>2) (0) (1) Part 3) After completion of all expected reactions, ratio of H ion concentration inside vs. outside the mitochondrial inner membrane should be? (<1) (~1) (>1)(0) Please provide brief explanationarrow_forwardWhy are estimates of ATP production from cellular respiration provided in terms of a range (30-32, for example)?arrow_forward
- Calculate the number of ATPATP generated from one saturated 1212‑carbon fatty acid. Assume that each NADHNADH molecule generates 2.5 ATP2.5 ATP and that each FADH2FADH2 molecule generates 1.5 ATP1.5 ATP .arrow_forwardConsider the following equilibrium at 298 K. R=8.314 J/K-mol Acetyl-CoA + Oxaloacetate + H20 Citrate + COASH + H* Which one of the following is the correct value for K'eg ifAG" = -32.2 kJ/mol? O 1.11 0.987 1.01 x 10-13 13.0 9.92 x 1012 2480 4.42 x 105 2.27 x 10-6 1.01 -2480 2.56 -13.0 1.08 -0.0130 0.0130 0.926arrow_forwardThe cytochromes are heme-containing proteins that function as electron carriers in the mitochondria. Calculate the difference in the reduction potential (AE°') and the change in the standard free energy (AG°) when the electron flow is from the carrier with the lower reduction potential to the higher. cytochrome c₁ (Fe³+) + e¯ = cytochrome c₁ (Fe2+) E°' = 0.22 V cytochrome c (Fe³+) + e¯ = cytochrome c (Fe²+) E°' = 0.254 V Calculate AE°' and AG°'. AE°' = AG°' = V kJ/molarrow_forward
- a) Assuming that ubiquinone is unavailable inside of the cell, calculate the AG and the Keq if electrons are transferred directly from complex I to complex III of the electron transport chain. In your answer include the net equation for this electron transfer. Constants: R= 8.3J/degree'mol, F= 96.1kJ/volt mol, T=298K ( Half Reaction EM ubiquinone + 2e + 2H' Ubiquinol + H2 NAD + 2e+ 2H'→ NADH + H 0.045 -0.320 1/202 + 2e +2H H,O Cytc (Fe") + +le + 1H"→ Cytc (Fe²) 0.816 0.254 b) In a single sentence explain whether or not this electron transfer is possible. /arrow_forwardCalculate the ΔG for Malate dehydrogenase reaction of CAC if the concentration of oxaloacetate is 1x 10-8 M, malate is 0.2 mM, NAD+ is 10 mM and NADH is 0.1 mM in rat liver mitochondria. ΔGo’ for this reaction is 30 kJ/mole. Please write the units of the final answer and the formula you are using to solve. R = 0.008314kJ/K*mol, T = 298Karrow_forward
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