Consider what would happen if you had a pure preparation of each of the substances below, which you added to an aqueous buffered solution (at physiological pH) and sonicated (mixed up). Which of the following would be most likely to spontaneously assemble into a micelle? H₂C HC-0 H₂C-O HO O O H₂C CH₂ H₂C-O-H 요 HC-O-C H₂N-CH₂-CH₂-O-P-0-CH₂ O=P-O CH₂ -NH3
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- At what pH does a lysine solution exhibit the highest buffering capacity? (Lysine pKas: pk1 = 2.2, pK2 = 8.95, pK3 = 10.5) %3D O рH 12.1 О рH 9.72 O pH 5.67 pH 8.95Which of the following is TRUE if pH is higher than the pka of the buffer? O [weak acid] approximately 0 O [conjugate base] = [weak acid] [conjugate base] > [weak acid] O [conjugate base] < [weak acid]The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.
- How much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)explain step by step why the correct answer is CSuppose you wanted to make a buffer of exactly pH 7.00 using KH2PO4 and Na2HPO4. If the final solution was 0.1 M in KH2PO4, what concentration of Na2HPO4 would you need?
- You have been observing an insect that defends itself from enemies by secreting a caustic liquid. Analysis of the liquid shows it to have a total concentration of formate plus formic acid (K₁ = 1.8 x 10-4) of 1.45 M. Further analysis reveals that the concentration of formate ion is 0.015 M. What is the pH of the secretion? pH =A glucosamine solution of unknown concentration was diluted as follows: 1 mL of the solution was diluted to 5.0 mL. A 0.3 mL aliquot of this solution was diluted with 0.7 mL of water; the absorbance of this was measured and found to be 0.440 (uncorrected, must subtract blank). What is the concentration of the original undiluted glucosamine hydrochloride solution in µmol/mL? Given: Corrected absorbance = 0.395 y= 1.165x+0.055 Standard equationConsider a mixture comprised of the proteins below: Protein that will most strongly bind to an anion exchange column is? Protein that will elute first in gel filtration chromatography is? Protein that will elute last in hydrophobic interaction chromatography is? Protein that will elute last in carbohydrate containing column is ?
- Define the following:- pH- Buffer- pKaMake 100 ml of a 1% solution of boric acid isotonic with blood by adding NaCl. The freezing point of 1% boric acid is -0.29°C. The freezing point of 1% sodium chloride is -0.58°C. What is the weight of sodium chloride (in grams) that must be added to the preparation to make it isotonic? * Your answerDextran is a polymeric carbohydrate produced by certain bacteria. It is used as a blood plasma substitute. An aqueous solution contains 0.582 g of dextran in 106 mL of solution at 21°C. It has an osmotic pressure of 1.47 mmHg. What is the average molecular mass of the dextran?