Catalase has a Km of 25 mM and a kcat of 4.0 x 107 s−1 with H2O2 as a substrate. Carbonic anhydrase has a Km of 26 mM and a kcat of 4.0 × 105 s−1. What do these data tell you about these two enzymes?

Q: Assume that an enzyme-catalyzed reaction follows the scheme shown: E + S ES E + P k₁ = 1 x 10%/M-s…

A: Michaelis Menten postulated that free enzyme reacts with the substrate reversibly to form an…

Q: In the microbial community of the bovine rumen, the actual AG value has been calculated for glucose…

A: Fermentation is an anaerobic metabolic process through which carbohydrates, such as glucose are…

Q: An enzyme catalyzes a reaction at a velocity of 20 micromole/min when the concentration of substrate…

A: The Michaelis-Menten equation represents the relationship between substrate concentration and…

Q: Staphylococcal nuclease has a ΔΔG‡ of -84.1 kJ mol-1 at 25.0 °C. If the uncatalyzed rate is…

A: We know that,ΔG = -RTlnKΔG = change in gibbs energy,when operated at 25 oC  = 25 +273 = 298K from…

Q: An enzymatic reaction with KM = 4.4 x 10-5 M, is carried out in 400 μL of of solution containing…

A: Given Values: Eo= 0.20 nmoles Vmax=6.6×10-3 M/s Km=4.4×10-5 M

Q: which staments are false

A: Note: Due to the length of the question and the fact that it combines numerous concepts and…

Q: Calculate the Km and Vmax for DADH with this substrate. Show your work.

A: Michaelis menten constant, Km is the substrate concentration required to produce half maximum…

Q: Explain why each of the following data sets from a Lineweaver–Burk plot are not individually ideal…

A: Lineweaver-Burk plot is the graphical representation of Lineweaver-Burk equation which is a double…

Q: The enzyme phosphoglucomutase catalyzes the conversion of glucose 1-phosphate to glucose…

A: Phosphoglucomutase is the enzyme that catalyze the interconversion between glucose 1-phosphate (G1P)…

Q: The KM for the reaction of chymotrypsin with Substrate A is 8.8 x 10-4 M, while the KM for the…

A: The Michaelis Menten constant (KM) can be used as a measure of the enzyme's affinity for its…

Q: The equil ibrium constant for the attachment of a substrate to the active site of an enzyme was…

A: The equilibrium constant of a chemical reaction is the value of its reaction quotient at chemical…

Q: In making wine, glucose (CH12O6) is fermented to produce ethanol (C;HSOH) and carbon dioxide (CO2)…

A: The wine is produced from raw materials that are rich in glucose such as hiney, grapes, pineapple,…

Q: Vmax and Km were calculated for enzyme substrate data. Results Y=0,0063x+0,3929 Km =62,4 mikromol/L…

A: From the LB plot of enzyme kinetics, y=mx+c  It's an equation of a straight line. 1/V=(Km/Vm )1/S…

Q: when saturated with substrate, an enzyme has a maximum initial rate of 110mumoles of substrate…

A: Asked : Reaction rate when substrate concentration is 2x10^-3M

Q: Most glucose in the human diet comes from plants. Unlike humans, plants transport sucrose instead of…

A: The reaction is in equilibrium if the forward and backward reaction is balanced.

Q: The complete combustion of palmitate and glucose yields 9781 kJ ∙ mol−1 and 2850 kJ ∙ mol−1 of free…

A: We are asked to find out which process is more efficient under physiological conditions( in vivo),…

Q: An enzyme catalyzes a reaction with a Km of 9.50 mM and a Vmax of 2.10 mM s-. Calculate the reaction…

A: Most of the enzymes follow Michalis-Menten enzyme kinetics. In this equation, Vmax is the maximum…

Q: The protein catalase catalyzes the reaction 2 H,O,(aq) — 2 H₂O(1) + O₂(g) S and has a…

A: Reaction: 2H₂O₂(aq) → 2H2O(l) + O2(g)Km = 25 mMTurnover number Kcat =  4.0 × 107s–1Total enzyme…

Q: If the Go for ATP hydrolysis into ADP + inorganic phosphate is 7.3 kcal/mole, and the Go for…

A: Introduction :- Inorganic phosphate (Pi) is a negatively charged ion that plays a key role in energy…

Q: Table 1 shows the kinetic data that have been obtained for glucoamylase from Aspergillus niger at…

A: Enzymes catalyse the biochemical reactions by decreasing the activation energy. Enzyme kinetics are…

Q: Both the yeast Saccharomyces cerevisiae and the bacterium Zymomonas mobilis produce ethanol from…

A: The production of ethanol through fermentation has gained significant attention due to its potential…

Q: Xanthine oxidase (XO) is the enzyme that catalyzes the synthesis of uric acid, which in excess buses…

A: Kaemferol inhibitory effect on Xanthine oxidase: Enzymatic assays have shown that kaempferol…

Q: (a) At what substrate concentration would an enzyme with a kcat of 30.0s−1 and a Km of 0.0050 M…

A: The enzyme are the biocatalysts that increase the rate of reaction by decreasing the activation…

Q: Given the following information about an enzymatic reaction, calculate the initial velocity. Your…

A: The Michaelis–Menten kinetics of an enzymatic reaction shows the relation between the substrate…

Q: Calculate the reduction potential of cytochrome a3 (°' = 0.385 V) at 25.0°C when the Fe3+ form =…

A: Biological oxidation-reduction reactions involve the transfer of electrons from one biomolecule,…

Q: With the help of the half reactions given in Table 1, formulate the redox equation for the oxidation…

A: FADH2 skips Complex I of the electron transport chain and donate the electron it is carrying to…

Q: The values ​​of the reduction potential (Eº') of the conjugated redox pairs NAD+/NADH and pyruvate /…

A: The significance of reduction potentials comes from the fact that when E has been determined for two…

Q: Using the graph paper provided, plot the velocity of the reaction (v) against the concentration of…

A: Michaelis Menten (MM) plot has [S] as X-axis and V as Y-axis. Here we are given the 1/[S] and 1/V…

Q: For a Michaelis-Menten enzyme, k1 = 5.2 ⅹ 108 M-1 s -1 , k-1 = 3.1 ⅹ 104 s -1 , and k2 = 3.4 ⅹ 105 s…

A: The initial Michaelis-Menten (MM) assumption that got postulated was that of rapid equilibrium. In…

Q: Consider the fructose-1,6-bisphosphatase reaction. Calculate the free energy change if the ratio of…

A: The objective of this question is to calculate the free energy change (ΔG) for the…

Q: The following question focuses on how the parameters regulating enzyme function might change, and…

A: Michaelis-Menten (MM) plot and Lineweaver Burk (LB) plot are drawn to decipher the kinetic…

Q: Suppose that you have isolated the enzyme sucrase (able to hydrolyze sucrose into glucose and…

A: Enzyme is a catalytic molecule that increases the rate of any chemical reaction without being used…

Q: The following data were collected for an enzyme that can be described by Michaelis Menton kinetics.…

A: In-order to get accurate Km and Vmax values, we need to draw the LB plot. The LB plot has 1V (i.e.…

Q: At what substrate concentration would an enzyme with a kcat of 33.0 s-1 and a Km of 0.0046 M operate…

A: The Michali s-Menten kinetics is enzyme kinetics that is common in biochemistry to detect and…

Q: Solve the Michaelis-Menten equation for KM when vo=Vmax/2. What does this tell you about the…

A: Enzymes catalyse biochemical reactions by lowering their activation energy.Enzymes have their…

Q: You are attempting to determine KM by measuring the reaction velocity at different substrate…

A: Michaelis-Menton constant or Km is the substrate concentration at which the reaction velocity is…

Q: You are working on an enzyme that obeys standard Michaelis-Menten kinetics. You have determined the…

A:

Q: An L-amino acid oxidase in mammalian peroxisomes requires H2O and O2 as substrates and catalyzes…

A: L-amino acid oxidase are flavoenzymes.

Q: Write a balanced equation for the oxidation of ubiquinol by cytochrome c. Calculate ΔG°′ and Δℰ°′…

A: Ubiquinol and cytochrome c are the components involved in the electron transport chain in…

Q: BAPNA ΒΑΡΝΑ Abs @410 [p-nitroanil [p-nitroanil nm (mm) 0 0.2625 0.525 1.05 2.1 4.2 (mm) 0 0.0656…

A: To plot the velocity of the reaction (v) against the concentration of BAPNA substrate ([S]) , we…

Q: The Vmax for a particular enzyme is 10 nmols/L/s. The Km for its substrate is 5 microM. If the…

A: Given that, the Vmax for a particular enzyme is 10 nmol/L/s. The Km for its substrate is 5 uM. We…

Q: In the hexokinase reaction (in glycolysis) Keq caclualtion, what is the OBVIOUS problem with getting…

A: Cellular respiration is a catabolic pathway of the process of metabolism, where a series of chemical…

Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.