C. You and your team of scientists continue to study zombin, with a particular emphasis on determining its function in these WBC. You've found that the zombin protein inhibits an enzyme called sapienase in humans. Sapienase normally functions to prevent immune cells (WBC) from attacking neurons, and therefore helps maintain normal brain activity and behavior. You perform a kinetic analysis of purified sapienase in vitro, both in the absence of purified zombin protein and in the presence of increasing concentrations of purified zombin protein (labeled as inhibitor, I). The results are shown below. Increasing [1] No inhibitor 1 1 1 Vo V, [S] V max max 1/[S] 1. What type of enzyme kinetics plot is shown above? 2. What is the advantage of using this type of plot versus the other type of enzyme kinetics plot we studied this semester? 3. What does Km measure? 4 How is the kKm volue for conienese offected by inereocing coneontrotione of zo mbin?
C. You and your team of scientists continue to study zombin, with a particular emphasis on determining its function in these WBC. You've found that the zombin protein inhibits an enzyme called sapienase in humans. Sapienase normally functions to prevent immune cells (WBC) from attacking neurons, and therefore helps maintain normal brain activity and behavior. You perform a kinetic analysis of purified sapienase in vitro, both in the absence of purified zombin protein and in the presence of increasing concentrations of purified zombin protein (labeled as inhibitor, I). The results are shown below. Increasing [1] No inhibitor 1 1 1 Vo V, [S] V max max 1/[S] 1. What type of enzyme kinetics plot is shown above? 2. What is the advantage of using this type of plot versus the other type of enzyme kinetics plot we studied this semester? 3. What does Km measure? 4 How is the kKm volue for conienese offected by inereocing coneontrotione of zo mbin?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
Please answer questions 1-3
![C. You and your team of scientists continue to study zombin, with a particular emphasis on determining its function in these WBC. You've found that the
zombin protein inhibits an enzyme called sapienase in humans. Sapienase normally functions to prevent immune cells (WBC) from attacking neurons,
and therefore helps maintain normal brain activity and behavior. You perform a kinetic analysis of purified sapienase in vitro, both in the absence of
purified zombin protein and in the presence of increasing concentrations of purified zombin protein (labeled as inhibitor, I). The results are shown below.
Increasing
[1]
No inhibitor
Km 1
[S]
V max
1
1
%3D
Vo
Vmax
1/[S]
1. What type of enzyme kinetics plot is shown above?
2. What is the advantage of using this type of plot versus the other type of enzyme kinetics plot we studied this semester?
3. What does Km measure?
4. How is the Km value for sapienase affected by increasing concentrations of zombin?
5. What type of inhibitor is zombin on sapienase?
6. To save the world from the zombie apocalypse, your team synthesizes a drug that prevents zombin from binding sapienase. Describe one possible
mechanism for how a drug might accomplish this task.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F1fc450b3-2147-46ae-beba-f01f47820724%2F03a15bb4-3b87-468e-9cf8-dc0c3dd5a73b%2Faoj7qll_processed.png&w=3840&q=75)
Transcribed Image Text:C. You and your team of scientists continue to study zombin, with a particular emphasis on determining its function in these WBC. You've found that the
zombin protein inhibits an enzyme called sapienase in humans. Sapienase normally functions to prevent immune cells (WBC) from attacking neurons,
and therefore helps maintain normal brain activity and behavior. You perform a kinetic analysis of purified sapienase in vitro, both in the absence of
purified zombin protein and in the presence of increasing concentrations of purified zombin protein (labeled as inhibitor, I). The results are shown below.
Increasing
[1]
No inhibitor
Km 1
[S]
V max
1
1
%3D
Vo
Vmax
1/[S]
1. What type of enzyme kinetics plot is shown above?
2. What is the advantage of using this type of plot versus the other type of enzyme kinetics plot we studied this semester?
3. What does Km measure?
4. How is the Km value for sapienase affected by increasing concentrations of zombin?
5. What type of inhibitor is zombin on sapienase?
6. To save the world from the zombie apocalypse, your team synthesizes a drug that prevents zombin from binding sapienase. Describe one possible
mechanism for how a drug might accomplish this task.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education