Based on this data, complete the table below: Plate # Dilution in Test Tube Inoculum Volume Titer (PFU's/ml) 1 2 3 4

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter4: Basic Immunology And Immunohematology
Section4.3: Tests For Rheumatoid Factors
Problem 2.1CS
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2. You have a sewage sample with an unknown concentration of viruses. You perform the
following serial dilution.
Iml
Iml
1ml
0. Iml
Iml
1ml
10ml
3
4
5
6.
7
999ml
9ml
9ml
99.9ml 9ml
9ml
990ml
Plaque assay 0.01 ml
0 1
2
3
4
6
7
You obtain the following plaque counts:
PLATE 0
1
2
3
TNTC
4
6
7
PFU
TNTC
TNTC
TNTC
256
30
4
TFTC
Based on this data, complete the table below:
Plate #
Dilution in Test Tube
Inoculum Volume
Titer (PFU's/ml)
1
3
4
7
Transcribed Image Text:2. You have a sewage sample with an unknown concentration of viruses. You perform the following serial dilution. Iml Iml 1ml 0. Iml Iml 1ml 10ml 3 4 5 6. 7 999ml 9ml 9ml 99.9ml 9ml 9ml 990ml Plaque assay 0.01 ml 0 1 2 3 4 6 7 You obtain the following plaque counts: PLATE 0 1 2 3 TNTC 4 6 7 PFU TNTC TNTC TNTC 256 30 4 TFTC Based on this data, complete the table below: Plate # Dilution in Test Tube Inoculum Volume Titer (PFU's/ml) 1 3 4 7
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