Bacterial colonies, each derived from a single cell Suspension of bacterial cells Suspension spread on petri plate with agar gel Incubate from 1 to 2 days Single cells with agar gel (not visible to naked eye) Visible colonies (each a clone of the corresponding single cell) Petri plate exhausted or until toxie tion growth. A small ar containing solid agar spreader, in a process they cannot travel far clump. When this mas naked eye as a colony. single original cell. Me FIGURE 5-3 Bacterial phenotypes can be assessed in their colonies. A stock of bacterial cells can be grown in a liquid medium containing nutrients, and then a small number of bacteria from the liquid suspension can be spread on solid agar medium. Each cell will give rise to a colony. All cells in a colony have the same genotype and phenotype. known as cell clones.

Aquaculture Science
3rd Edition
ISBN:9781133558347
Author:Parker
Publisher:Parker
Chapter2: Aquatic Plants And Animals
Section: Chapter Questions
Problem 2KA
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In Figure 5-3, if the concentration of bacterial cells in
the original suspension is 200/ml and 0.2 ml is plated
onto each of 100 petri dishes, what is the expected average number of colonies per plate?

Bacterial colonies, each derived from a single cell
Suspension of
bacterial cells
Suspension spread on
petri plate with agar gel
Incubate from
1 to 2 days
Single cells
with agar gel (not visible
to naked eye)
Visible colonies
(each a clone of
the corresponding
single cell)
Petri plate
exhausted or until toxie
tion growth. A small ar
containing solid agar
spreader, in a process
they cannot travel far
clump. When this mas
naked eye as a colony.
single original cell. Me
FIGURE 5-3 Bacterial phenotypes can
be assessed in their colonies. A stock of
bacterial cells can be grown in a liquid
medium containing nutrients, and then a
small number of bacteria from the liquid
suspension can be spread on solid agar
medium. Each cell will give rise to a
colony. All cells in a colony have the same
genotype and phenotype.
known as cell clones.
Transcribed Image Text:Bacterial colonies, each derived from a single cell Suspension of bacterial cells Suspension spread on petri plate with agar gel Incubate from 1 to 2 days Single cells with agar gel (not visible to naked eye) Visible colonies (each a clone of the corresponding single cell) Petri plate exhausted or until toxie tion growth. A small ar containing solid agar spreader, in a process they cannot travel far clump. When this mas naked eye as a colony. single original cell. Me FIGURE 5-3 Bacterial phenotypes can be assessed in their colonies. A stock of bacterial cells can be grown in a liquid medium containing nutrients, and then a small number of bacteria from the liquid suspension can be spread on solid agar medium. Each cell will give rise to a colony. All cells in a colony have the same genotype and phenotype. known as cell clones.
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