At blood pH 7.4 the molar ratio of bicarbonate and dissolved CO2 is: Group of answer choices 20 to 1 4 to 1 1 to 4 1 to 20When using an Indirect Ion Selective electrode: The measurement will not suffer interferences from lipids The measurement will not suffer interferences from proteins The sample will be analyzed without a dilution The sample will be analyzed after a dilution
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At blood pH 7.4 the molar ratio of bicarbonate and dissolved CO2 is: Group of answer choices 20 to 1 4 to 1 1 to 4 1 to 20When using an Indirect Ion Selective electrode: The measurement will not suffer interferences from lipids The measurement will not suffer interferences from proteins The sample will be analyzed without a dilution The sample will be analyzed after a dilution
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- 18 Students were provided with cubes of slightly alkaline gelatine of different dimensions, containing an acid-alkali indicator that is red in alkali but yellow in acid. The cubes were placed in dilute acid solution, and the time taken for the colour in the gelatine to change from red to yellow was measured. Dimensions/mm Surface area/mm² Volume/mm³ Time/minutes 10 x 10 x 10 600.0 1000.0 12.0 5x 5 x5 150.0 125.0 4.5 4 x 4 x 4 96.0 64.0 4.2 2.5 x 2.5 x 2.5 a For each block, calculate the ratio of surface area to volume (SA:V). b Plot a graph of the time taken for the colour change (vertical or y axis) against the SA:V ratio (horizontal or x axis). c Explain why the colour changes more quickly in some blocks than others. 37.5 15.6 4.0Volume Base: Additives Concentration Potassium Acetate 2 mEq/mL Dose Ordered Volume (mL) mL 30 mEq 12.5 mL Sodium Chloride 4 mEq/mL 50 mEq mL Ca Gluconate 0.465 mEq/mL 8 mEq 322 10 mL MVI-12 10 mL mL Famotidine 10 mg/mL 40 mg LI5. mL Volume Additives: mL Final Volume: Calculate the amount of Sterile Water for Injection needed to qs the total volume to 1,000 mL. mL Sterile Water: mL How many mLs should you remove from your Dextrose 70% bag? ccess Code Inside ources on EvolveThe pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if HCl in pH electrode, became 0.15M instead of 0.1M ? a.impact can not be determined b.measured pH is higher than "real" pH. c.measured pH is lower than "real" pH. d.measured pH is same as "real" pH.
- The before and after fluids were observed to detect the movement of starch across the dialyzing membrane. The tables below shows the test results using chemical indicators like iodine test. BEFORE Fluid in the beaker yellow (absence of starch) Fluid in the bag black (presence of starch) AFTER Fluid in the beaker yellow (absence of starch) Fluid in the bag black (presnece of starch) Was there a movement of starch across the dialyzing membrane? Why or why not?Solution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…
- Match the common laboratory scenarios to the correct buffer required using the guide below. Molecule pKa Value(s) Acetic acid 4.8 Carbonic acid 6.1, 10.2 Citric acid 3.1, 4.8, 5.4 HEPES 7.5 Phosphoric acid 2.1, 7.1, 12.3 TRIS 8.3 Mammalian cells require a pH of about 7.4 for cell and tissue culture experiments. Elution of remaining molecules from a cation exchange column requires washing with pH 10 buffer. Dissolution of lysozyme requires pH 3 buffer to maintain activity. Elution of remaining molecules from an anion exchange column requires washing with pH 2 buffer.…Match the following descriptions to the tonicity adjusting method which they describe. - Cryoscopic method - White-Vincent Method - E method - Sprowl's method It is the most widely used method in adjusting tonicity by the amount of sodium chloride. This method expresses the freezing point in terms of 1% solution. This method uses a constant that denotes the volume of isotonic preparation in milliliters when 1 gram of sodium chloride is dissolved in water. This method uses a constant of 0.3 to denote the weight of the drug. This method uses the equation "V = 33.33E".Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…