Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
- Aspergillus can be used to produce α-amylase (α-AM), which is an enzyme that hydrolyzes the glycosidic bonds in starch molecules into maltose (disaccharide). Pure α-AM has a specific activity of 56.00 U/mg. The α-AM is secreted into the culture media during production (after 5 days, production is complete). Following, the Aspergilluscells are separated by centrifugation and discarded. The culture supernatant (with α-AM) is filtered giving a clarified supernatant with a volume of 100.0 L, activity of 3.00 U/mL, and total protein concentration of 0.650 mg/mL. After purification, the product stream (7.50 L) contains α-AM (25.00 U/mL) and a total of 6.788 x 103 mg total protein. Calculate the following:
- Concentration of total protein (g/L) and total units of activity in the clarified supernatant and purification product stream.
- Specific activities (U/g) of the clarified supernatant and purification product stream. Consider significant figures in your answers (may want to use scientific notation to assist).
- α-AM purities in the clarified supernatant and purified product stream.
- Fold-purification.
- Concentration factor and yield of α-AM for the purification step.
Expert Solution
arrow_forward
Step 1
Alpha amylase is an enzyme that hydrolyzes the alpha-1,4 glycosidic bonds in starch molecules, breaking them down into smaller carbohydrates. This enzyme is widely used in various industries, including food, brewing, and biofuel production. It is produced by several microorganisms, including Aspergillus, and has a specific activity that can be measured in units per milligram.
Trending nowThis is a popular solution!
Step by stepSolved in 2 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Draw the products formed when β-D-galactose is treated with below reagent. NaH + C6H5CH2Clarrow_forwardBased on only the given information, which organism is likely most resistant to thermal sterilization? Org X, Z-value of 10.0 °C Org Y, Z-value of 2.0 °C Org Z, a mesophile Org K, a psychrotropharrow_forwardWhich would be the control plate for the NaCl experiment and the Sucrose experiment and why?arrow_forward
- A 250mL solution of penicillin and streptomycin needs to be made from penicillin G powder and streptomycin sulfate powder. Can you explain how to make this? Please show details, thank you.arrow_forwardO The following is a Mannitol Salt Agar plate. What does the growth on this plate indicate? www TAN ASM's MicrobeLibrary Reynolds lactose metabolism tolerance of the dyes and chemicals in the agar tolerance of the salt mannitol metabolismarrow_forwardIdentify the two structures labeled as the number four in the image belowarrow_forward
- What Organism could this be?arrow_forwardWhat is "BHI" agar and what is it used for? What is "Sabouraud Dextrose" agar and what is it used for?arrow_forwardBetween Bacillus megaterium, Bacillus sphaericus, and Bacillus subtilis which are positve and which are negative for oxidase test results?arrow_forward
arrow_back_ios
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education