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What is the purpose of the crystal violet, bile salts, and neural red in MacConkey Agar media?
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- The principle and What type pf Media of the following Conkey Agar vii) MacConkey Broth viii) TSA Base for Blood and chocolate Agar ix) Mannitol Salt Agar x) Tryptic Soy Agar xi) Alkaline peptone water What is the principle of Growth Medium and Culture medium?What is "BHI" agar and what is it used for? What is "Sabouraud Dextrose" agar and what is it used for?why macconkey agar and mannitol salt are regard as salective and deffrental medium?
- Why does Propionigenium modestum require sodium for growth?Examine the graph shown below of a liquid culture growth curve for S. pneumoniaegrowing in minimal broth containing both glucose and lactose in equal concentrations as a potential carbon source. Answer the following questions: a) Name the growth phases labelled as I, II and III, explain the observed pattern of growth at each of these stages in the curve b) Where would the concentration of cAMP be low and why? c) In what phase would β-galactosidase be high and why? d) What kind of overall growth pattern does this curve illustrate?why is it important that a small amount of inoculum be used in preparation of an agar slant?
- What is the major difference between Thayer-Martin and chocolate agar? When would you use Thayer-Martin rather than chocolate agar?1(a) 1. What is the theory upon which intermittent sterilization is based? (B) Is dry or moist heat more efficient as a sterilizing agent? Why? (C) When would it be necessary to use aseptic techniques when inoculating a tube of nutrient broth with dirt? (D)Why do some microorganisms characteristically form a growth of the pellicle type? What environmental factors might alter the formation of a pellicle? (This is not a graded assignment)What do pink to dark red colonies with a metallic surface sheen indicate on Endo agar?