Which would be the control plate for the NaCl experiment and the Sucrose experiment and why?

Give typing answer with explanation and conclusion  Why 70% Alcohol is better than 100% Alcohol (as an antiseptic) to control the growth of bacteria?

Explain briefly why choosing a specific standard is less important for determining the concentration of protein in the cell lysate compared to the elution fraction?

You set up the following two tubes: Tube 1: 1mL of alkaline phosphatase + 4mL of 400 µM p-NPP Tube 2: 1mL of alkaline phosphatase + 4mL of 200 µM p-NPP Which tube would have a faster reaction rate? Explain your reasoning (why does concentration of p-NPP matter? Relate it back to week 4 lab concepts).   Week 4 lab concepts:  - concentration gradient - differentially permeable membrane - solute, solvent, diffusion, osmosis, hypotonic, isotonic, hypertonic, molarity, osmolarity

What is meant by the statement “orthogonal analysis methods should be used for characterization of impurities”? Why do bacterial and plant cells require partial destruction prior to osmotic lysis? How can the partial destruction be achieved?

What reaction would you expect when performing a positive control in the oxidase assay? What would it mean if a known oxidase-positive bacterium did not cause the expected reaction?

During the Ames test, his− bacteria are exposed to a mixture of chemical compounds extracted from SurbWay Sandwich. If the his− bacteria revert to his+, then what can we say about the chemical?

How would you set up an assay to measure the activity of an enzyme, such as catalase, in a tissue extract?

Why is there a difference in the way these two kinds of cells react in a hypotonic solution?

When you graph your protein assay data in Excel, should you include the absorbance of your unknown BSA sample? Explain why or why not