Asolution of double-stranded DNA in 0.2M NaCI gives an A260 of 20 with an extinction coefficient of 20 g'em-'L and a spectrometer path length of lem. What is the concentration oft he solution?

Quantity of total DNA in a 5ul sample: Absorbance at 260nm 0.002 and at 280nm = 0.0011

The bloud trans fusion is Infusing af 25 drups per mirute, and the Set is calibrated at 15 drops/ml. what is the hourly Infugim rate In mililitres ?

What does -log(p-value) tell in immunoprecipitation mass spectrometry? What does log2 Fold change say? Generally, what does the graph of immunoprecipitation mass spectrometry tell (ie. how to read its graph)?

During in vitro synthesis of DNA, a reasercher used 2',3'-didoxy cytidine triphosphate as raw neclutide in place of 2'deoxy cytidine.what woulb be consider?

V2

EcoRI 1.1kbp 1.5 kbp Hindll Hindll 0.15 kbp amp 0.25 kbp EcoRI If you were to digest this plasmid with HindllI, how many fragments would be visible using gel electrophoresis? O 1 O 4

Deinococcus radiodurans, which the Guinness Book of World Records has dubbed the world’s toughest bacterium, can tolerate doses of ionizing radiation ∼3000-fold greater than those that are lethal to humans (it was fi rst discovered growing in a can of ground meat that had been “sterilized” by radiation). It appears to have several strategies to repair radiation damage to its DNA (which large doses of ionizing radiation fragment to many pieces) including a particularly large number of genes encoding proteins involved in DNA repair and 4 to 10 copies per cell of its genome, which consists of two circular chromosomes and two circular plasmids. Yet, these strategies, alone, do not account for D. radiodurans’ enormously high radiation resistance. However, in an additional strategy, it organizes its multiple identical dsDNA circles into stacks in which, it is thought, the identical genes in the neighboring circles are aligned side by side. How would this latter strategy help D. radiodurans…

Draw a dilution scheme for a dilution of phage from 1.5 x 1010 PFU/mL to 1.00 x 102 PFU/mL. Achieve this serial dilution considering the following: The volumes of each dilution should be 1.0mL. It is not advisable to perform any dilutions > 1/100. The first dilution in any titre determination of stock phage should use 10µl or less of stock.