Alternative splicing a. increases the number of proteins made from one gene b. increases the number of primary transcripts made from one gene c. increases the number of exons d. increases the number of introns

Cystic fibrosis is a life-threatening disease that causes thick, sticky mucus to build up in areas of the body, including the lungs. Scientists are studying CFTR, the gene associated with cystic fibrosis in humans. In the majority of cystic fibrosis patients, the same mutation is seen in the same location on the CFTR geně. A partial DNA sequence, a partial MRNA transcript, and part of the normal polypetide that the makes up the CFTR protein that the CFTR gene codes for are shown in the diagram below. DNA 3 MRNA 5 AUC Polypeptide A partial DNA sequence, a partial MRNA transcript, and part of the CFTR polypeptide in a cystic fibrosis patient are shown in the diagram below. DNA 3 CACAA MRNA 5 Polypeptide Gly A Describe the change in the DNA sequence, the effect on the MRNA, and the effect on the polypeptide. B. Is this a "true" frameshift mutation? Why or why not? C. Would a single base insertion at the beginning of the gene have a larger or smaller effect on the resulting polypeptide…

When the spliceosome binds to the primary transcript shown above, it can attach Select one: O a. to the exons O b. to the 5' UTR O c. to the 3' UTR O d. at certain sites along an intron

In considering the 7 levels of gene regulation, which of the following mechanisms acts at first level? a. repressor binding to sequence blocking the promoter b. miRNA silencing c. differential processing of RNA d. protein modification e. answer may vary depending if this occurs in a prokaryote or a eukaryote

Which of the following will lead to a decrease in transcription of a gene? A. Increase in histone acetyltransferases. B. Decrease in CpG methylation of promoter. C. Increase in histone deacetylases. D. Decrease in DNA methyltransferases.

Alternative RNA splicing a. is a mechanism for increasing the rate of transcription. b. can allow the production of proteins of different sizes from a gene. C. can allow the production of similar proteins from different RNAS. d. increases the rate of transcription. e. is due to the presence or absence of particular snRNAs.

After treating cells in tissue culture with an HDAC inhibitor, which of the following is the most likely outcome? A. Repression of transcription B. Promoter methylation C. Enhanced splicing D. Activation of transcription E. Depends on the cell type

Choose all items that regulate the transcription of mRNAs.Group of answer choices A. Transcription factor proteins B. Intron sequences C.. DNA promoter sequences D. DNA enhancer regions E. Exon sequences

You need to determine if a specific mRNA is modified. What method will you choose to determine this and why?

Which of the following about the splicing in posttranscriptional processing is NOT true? a. In bacterial, the genes coding for polypeptides are interrupted by non-coding regions. O b. O C. O d. Introns in eukaryotic hnRNA must be cut out before mature mRNA can be used for protein synthesis. Alternate splicing can lead to tissue-specific proteins. Splicing can lead to multi-domain proteins.