ACH ACH + ACH + ACH + 1 x 10-M 3 x 10-M Acetylcholine Alone Concentration [Molar] Homatropine Homatropine Homatropine 3 x 10-⁹M 3 x 109 3 +/- 1 6 x 10-9 8 +/- 3 No response 3 x 10-8 28 +/- 6 10 +/- 4 No response 6 x 10-8 38 +/-8 21 +/-9 5+/-2 No response 3 x 10-7 55 +/- 5 43 +/- 7 27 +/-9 10 +/- 4 6 x 107 56 +/- 5 52 +/-3 38 +/- 7 20 +/- 7 3 x 10-6 55 +/- 4 53 +/- 6 43 +/- 6 6 x 106 56 +/- 2 54 +/- 5

The substances in the table below were chromatographed on a gel filtration column. Estimate the molecular weight of the unknown. Compound Blue Dextran 2000 Molecular weight 2 x 106 158 000 V. (mL) 17.7 Aldolase 35.6 Catalase 32.3 210 000 Ferritin 28.6 440 000 Thyroglobulin 25.1 669 000 Unknown 30.3

Below are responses generated using the isolated guinea-pig ileum preparation to determine a pA2 value for the cholinergic antagonist homatropine.Responses are expressed in millimetres +/- SDM (n = 4).1. Plot the data on a sheet of paper and then determine by Schild analysis the pA2 value and Schild slope 2. show all calculation on a table form on a sheet of paper 3. discuss the result with conclusion

Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.211   and A650=0.086, taking into account the dilution factor as per question 6 (100ul), and the total volume of extract as per question 4 (140ml) . Note your answer to 2 decimal places

If the endpoints you reached during standardization of your NaOH solution were very precise (relative average deviation if 0.03%) but were all overshot (meaning too much NaOH was added an you passedyour equivalence point), is the reported molar concentration of the acid solution from this experiment greater than , less than or equal to the true concentration? Explain.

(4) Below are depictions of a student's TLC plates in which fractions 1 - 10 from their column are spotted. Lane 1 2 3 4 5 67 8 9 10 (a) Which fractions contain pure fluorenone? Which contain pure ferrocene? (b) Which lanes, if any, represent mixed fractions? (c) Would the student incorporate lanes 6 and 7 into an Erlenmeyer flask of a combined fraction? Explain with 10 words or less.

Using the Chromatographs containing the unknown mixture of Acetates and the corresponding relative response factors from the standard mixture, calculate the mole percentage of Peak 2 in the unknown mixture.

2. Prepare a Temperature vs Time cooling curve for each of the two trials involving the lauric acid/stearic acid mixtures. Perform a linear regression analysis on the cooling and freezing regions as before. Calculate the point of intersection and identify this point on each graph by inserting a text box and arrow. Report the freezing point for each of the two trials [T(1) and T (2)]. Freezing point (1st trial of lauric acid) T(1) = Freezing point (2nd trial of lauric acid) T(2) =

2. Thin layer chromatography was performed to find the most effective mobile phase needed to separate a mixture containing three compounds (using silica gel as the stationary phase). TLC analyses were performed using five different mobile phases: (A) pure petroleum ether; (B) 25:75 ethyl acetate:petroleum ether; (c) 50:50 ethyl acetate:petroleum ether (D) 75:25 ethyl acetate:petroleum ether; and (E) pure ethyl acetate. Using the TLC results provided, indicate whether an effective separation was achieved for each of the five mobile phases. Be sure to provide a brief explanation for your answer and identify which mobile phase(s) could be used to offer an effective separation of this mixture using column chromatography. A D E