
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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Question
Below are responses generated using the isolated guinea-pig ileum preparation to determine a pA2 value for the cholinergic antagonist homatropine.
Responses are expressed in millimetres +/- SDM (n = 4).
1. Plot the data on excel and then determine by Schild analysis the pA2 value and Schild slope
2. show all calculation on a sheet of paper
3. discuss the result with conclusion
![ACH
ACH +
ACH +
ACH +
1 x 10-M
3 x 10-M
Acetylcholine Alone
Concentration
[Molar]
Homatropine Homatropine Homatropine
3 x 10-⁹M
3 x 109
3 +/- 1
6 x 10-9
8 +/- 3
No response
3 x 10-8
28 +/- 6
10 +/- 4
No response
6 x 10-8
38 +/-8
21 +/-9
5+/-2
No response
3 x 10-7
55 +/- 5
43 +/- 7
27 +/-9
10 +/- 4
6 x 107
56 +/- 5
52 +/-3
38 +/- 7
20 +/- 7
3 x 10-6
55 +/- 4
53 +/- 6
43 +/- 6
6 x 106
56 +/- 2
54 +/- 5](https://content.bartleby.com/qna-images/question/c504be15-4a2e-43e2-bac4-44543a772361/fe88fa5b-cf58-43e0-a6a7-b352f8985b6c/357gmop_thumbnail.png)
Transcribed Image Text:ACH
ACH +
ACH +
ACH +
1 x 10-M
3 x 10-M
Acetylcholine Alone
Concentration
[Molar]
Homatropine Homatropine Homatropine
3 x 10-⁹M
3 x 109
3 +/- 1
6 x 10-9
8 +/- 3
No response
3 x 10-8
28 +/- 6
10 +/- 4
No response
6 x 10-8
38 +/-8
21 +/-9
5+/-2
No response
3 x 10-7
55 +/- 5
43 +/- 7
27 +/-9
10 +/- 4
6 x 107
56 +/- 5
52 +/-3
38 +/- 7
20 +/- 7
3 x 10-6
55 +/- 4
53 +/- 6
43 +/- 6
6 x 106
56 +/- 2
54 +/- 5
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