For an enzymatic assay, tumour cells are incubated at different concentrations in individual vials (Table 1). Please note that the final reaction volume differs between the three setups. Vial No. A (ml) В (ml) C (ml) H20 1 Tris/HCl buffer pH7 1 1 1 Tumour cells (5% (w/v)) in buffer 1 3 4 Glucose [25mM] 0.5 0.75 1 Table 1. a) Calculate the weight (in gram/ml) of tumour cells in each vial. Show your workings. b) Calculate the glucose concentration in each vial (in mol/ml). Show your workings.
For an enzymatic assay, tumour cells are incubated at different concentrations in individual vials (Table 1). Please note that the final reaction volume differs between the three setups. Vial No. A (ml) В (ml) C (ml) H20 1 Tris/HCl buffer pH7 1 1 1 Tumour cells (5% (w/v)) in buffer 1 3 4 Glucose [25mM] 0.5 0.75 1 Table 1. a) Calculate the weight (in gram/ml) of tumour cells in each vial. Show your workings. b) Calculate the glucose concentration in each vial (in mol/ml). Show your workings.
Chapter28: Atomic Spectroscopy
Section: Chapter Questions
Problem 28.13QAP
Related questions
Question
![For an enzymatic assay, tumour cells are incubated at different concentrations in individual
vials (Table 1). Please note that the final reaction volume differs between the three setups.
Vial No.
А (ml)
В (ml)
C (ml)
H20
1
2
5
Tris/HCl buffer pH7
1
1
1
Tumour cells (5%
1
3
4
(w/v)) in buffer
Glucose [25mM]
0.5
0.75
1
Table 1.
a) Calculate the weight (in gram/ml) of tumour cells in each vial. Show your workings.
b) Calculate the glucose concentration in each vial (in mol/ml). Show your workings.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe4b2eb66-6876-46cd-8986-470643caa28d%2F0b4fe58a-3199-4948-ab57-916b1614ba4c%2Ff4a7sqq_processed.jpeg&w=3840&q=75)
Transcribed Image Text:For an enzymatic assay, tumour cells are incubated at different concentrations in individual
vials (Table 1). Please note that the final reaction volume differs between the three setups.
Vial No.
А (ml)
В (ml)
C (ml)
H20
1
2
5
Tris/HCl buffer pH7
1
1
1
Tumour cells (5%
1
3
4
(w/v)) in buffer
Glucose [25mM]
0.5
0.75
1
Table 1.
a) Calculate the weight (in gram/ml) of tumour cells in each vial. Show your workings.
b) Calculate the glucose concentration in each vial (in mol/ml). Show your workings.
Expert Solution
![](/static/compass_v2/shared-icons/check-mark.png)
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 3 steps with 2 images
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Recommended textbooks for you