The substances in the table below were chromatographed on a gel filtration column. Estimate the molecular weight of the unknown. Compound Blue Dextran 2000 V. (mL) 17.7 Molecular weight 2 x 106 Aldolase 35.6 158 000 Catalase 32.3 210 000 Ferritin 28.6 440 000 Thyroglobulin 25.1 669 000 Unknown 30.3

Briefly explain how analytes are separated using the following modes of chromatography.   Adsorption chromatography    Partition chromatography    Molecular exclusion chromatography               Affinity chromatography    Ion-exchange chromatography

Classify the descriptions based on whether they apply to ion‑exchange, size‑exclusion, or both types of chromatography columns. - Proteins move through the column at rates determined by their net charge. - The gel bed will contain polymer beads with charged functional groups. - Proteins move through the column at rates determined by their size. - The gel bed will contain porous polymer beads. - The properties of the proteins affect the rate at which they migrate. - Proteins are separated based on differences in their amino acid sequences.

Please propose a chromatography-based strategy to purify human insulin proteins that have been expressed in bacterial cells.