Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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- You are supplied with the following: / Jy word voorsien van die volgende: NaCl (Mr= 58,443 g /mol) 2.5M Tris-Cl, pH 8 solution /oplossing (1 Litre) EDTA (Mr= 380,2g/mol) 10% sodium dodecyl sulphate solution / natriumdodecyl sulfaat oplossing Proteïnase K solution / oplossing (50 mg dissolved / opgelos in 1 ml ddH2O) You need a digestion buffer consisting of the following: / Jy moet 'n verteringsbuffer op maak wat uit die volgende bestaan: 15m M NaCl 75 mM Tris-Cl,pH 8 16 mM EDTA,pH 8 0.8% sodium sulphate / natrium dodecyl sulfaat 0,75 mg/ml proteïnase K How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations. Remember to explain exactly how you will make it up. / Hoe sal jy 500 ml van die verteringsbuffer voorberei? Wys al jou stappe en berekeninge. Onthou om te verduidelik presies hoe jy dit sal op maak.arrow_forwardIf the osmolarity of a solution of NaCl is 2.4 OsM, what would it be as a percent solution? MW of NaCl = 58.44 g/molearrow_forward
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