Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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- Calculate the pH of a solution if [H3O+] = 3.4 x 10-2M Indicate whether the solution is acidic, basic, or neutralarrow_forwardThe amino acid glycine is often used as an ingredient in buffers for biochemistry experiments. The amino group of glycine has a pKa of 9.6. Glycine exists in either a protonated form (-NH3+) or a free base (-NH₂). a) In what pH range can glycine be used as an effective buffer? b) In a 0.1M solution of glycine at pH 9.0, what fraction has its amino group in the protonated form? c) When 99% of the glycine is in the protonated from, what is the numerical relation between the pH of the solution and the pKa of the amino group?arrow_forwardOnly 15-14arrow_forward
- 80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pl. The solution is then titrated with 0.2M HCI to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCl needed to reach each relevant pKa value and equivalence point(s). Relevant pka values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.arrow_forwardA monoprotic weak acid, HA, dissociates in water according to the reaction HA(aq) = H+ (aq) + A¯(aq) The equilibrium concentrations of the reactants and products are [HA] = 0.220 M, [H+] = 3.00 × 10−4 M, and [A¯] = 3.00 × 10−4 M. Calculate the value of pKa for the acid HA. pKa =arrow_forwardQuinine ( C20 H24 N2 O2) is the most important alkaloid derived from cinchona bark. It is used as an antimalarial drug. For quinine, pK, = 5.1 and pK, = 9.7 ( pKp = – log Kp). Only 1 g quinine will dissolve in 1920.0 mL of solution. Calculate the pH of a saturated aqueous solution of quinine. Consider only the reaction | Q+ H2O= QH+ + OH- described by pK, where Q = quinine. pH =arrow_forward
- What is the pH of a 0.150 M solution of acetic acid (pK₂ = 4.75) at 25 °C?arrow_forwardThe pOH of a basic solution is 3.75. What is [H⁺]?arrow_forwardpH of solution 14.00 12.00 10.00 8.00 6.00 First 4.00 equivalence point 2.00 0 First midpoint Second equivalence point Third midpoint pH = pKa=12.32 Third equivalence point HPO4(aq) + OH(aq) PO4(aq) + H2O(1) Second midpoint pH = pKa = 7.21 H2PO4 (aq) + OH(aq) HPO42 (aq) + H2O(1) Using the Henderson- Hasselback equation, show how to create 2L of a 0.1 M KPhos pH 7.5 buffer using K2HPO4 and KH2PO4. The chart to the left should help you understand what pKa to start with. Show your work. pH = pKa = 2.16 H3PO4(aq) + OH(aq) H2PO4(aq) + H2O(l) 25.0 50.0 75.0 100.0 Volume of NaOH added (mL)arrow_forward
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