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- Are the cells grown in the Laboratory will function similarly when transplanted? Cite some examples.About the technique of phage display: MOLECULAR BIOLOGY_advanced The Escherichia coli cell infected by the phage codifies for the optimized ligand when the phage DNA integrates in the DNA of the bacteria. Phages are selected if they express on their surface the optimized ligand. One selects Escherichia coli cells that are resistant to the phage infection. More than one optimized ligand can be selected during the panning procedure. The ligand to be selected on the surface of the phage is non-covalently linked to one of the surface proteins.Fluorescence in-situ hybridisation (FISH) analysis can be performed on fixed pathological tumour sections. Briefly outline why interphase FISH is used on fixed material from a solid tumour, rather than metaphase FISH
- Following is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…Are cells grown in the laboratory will function similarly when transplanted?Explain why gene mapping is important and how this technique can be used in diseaseinvestigation? (Answer within 250-500 words only)
- what technique was used to figure out if some Tasmanian devils would be more likely to survive the transmissible cancer reducing their numbers? - RNA-seq - GWAS - de novo sequencing - deep sequencingArrange the best steps to take to identify the gene and mutation that causes the lung cancer. Not all of the answers will be placed. Isolate tumor DNA lightly fragment the genome transfect the genome into mouse cell line determine shared transfected region repeat with small fragments and sequence the gene generate a cell line from the tumor sequence the tumor-cell genome isolate transformed colonies introduce transfected cells into a mouse Introduce the mutation in cell cultureIf graft qnd corruption are diseases, how do you propose to ameliorate their ravages? If they are only symptoms, what is the disease and how do you propose to treat it?
- Explain ORF analysis.In relation to immunotechnology, answer the following: A- Answer the following in relation to Hybridoma technology: → What is the cancer cell line name used in hybridoma technology?Describe what will happen to bacteria with dysfunctional restriction modification system providing that restrictase (first scenario) or methylase (second scenario) is not functional when it gets attacked by phage or by another bacterial species. T: BI