A nurse colleague asks for your help in drawing blood cultures from a patient with suspected bacteremia. You notice that he has handed you two bottles, each with a different colour label. What is the reason for having two different culture bottles? Question 17 options: a) One bottle is used to identify Gram positive organisms and the other bottle helps identify Gram negative organisms b) There is no reason for two bottles. The microbiology laboratory only needs one bottle c) One bottle is used for aerobic bacteria and the other bottle is used for anaerobic bacteria d) One bottle is used to identify the bacterial organism, and the other bottle is used as a back-up in case the microbiology laboratory needs a larger sample

Question 15 Which of the following characteristics is NOT used in the Baltimore classification system? A) Sense of strand Nucleic acid type Number of strands D Viral capsule morphology Pri Last Ter Type here to search 11 DII PrtScn F8 Esc F5 F6 F7 F1 F2 F3 F4 @ #3 24 & 5 6.

Question 18 The presence of squamous epithelial cells in an expectorated sputum sample indicates that: Question 18 options: a) The sputum was collected from deep in the lung b) The sputum is an early AM sample c) The sputum contains many pus cells d) The sputum sample consists mainly of saliva and should be rejected

QUESTION 2 The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral protein (current infection). You plan to express the mutant spike protein variant using the plasmid pictured below as your template. Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy as a gene block! But you need to add a couple things to it first so it'll get into the plasmid. To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867). Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the…