A new method is proposed to simultaneously detectand separatefour foodadditives by reversed-phase liquid chromatography. The data were obtained using theliquid chromatographic column with a flow rate of 0.5 mL per min and with the followingparameters: Pore size  100 A Particle Size 3.5  Internal diameter 4.6 mm Length 150 mm The food additives found in the sample resulted in the following data: ( see image for table)  a.Calculate the number of plates from each peak. b.Calculate the plate height for the column. c.Retention factor for each peak.

Fundamentals Of Analytical Chemistry
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ISBN:9781285640686
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Chapter16: Applications Of Neutralization Titrations
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A new method is proposed to simultaneously detectand separatefour foodadditives by reversed-phase liquid chromatography. The data were obtained using theliquid chromatographic column with a flow rate of 0.5 mL per min and with the followingparameters:

Pore size  100 A
Particle Size 3.5 
Internal diameter 4.6 mm
Length 150 mm

The food additives found in the sample resulted in the following data: ( see image for table) 

a.Calculate the number of plates from each peak.

b.Calculate the plate height for the column.

c.Retention factor for each peak.

 

The food additives found in the sample resulted in the following data:
Retention time (min) Width at peak base
1.05
Caffeine
Aspartame
Benzoic acid
Sorbic acid
5.560
9.787
12.053
2.65
4.00
2.58
18.511
3.96
Transcribed Image Text:The food additives found in the sample resulted in the following data: Retention time (min) Width at peak base 1.05 Caffeine Aspartame Benzoic acid Sorbic acid 5.560 9.787 12.053 2.65 4.00 2.58 18.511 3.96
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