Explain shortly how the chromatography will be influenced in a C18 reverse phase liquid chromatography column when: a) a 100 meter column with 20 000 theoretical plates is used in comparison to a 30 meter column with 25 000 theoretical plates. b) the flow rate is decreased. c) the temperature of the mobile phase is increased. d) the particle size of the stationary material is decreased. e) the particle shape of the stationary material is made more uneven. f) decreasing the column temperature.

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Q: What are the properties that determine retention time in the Chromatography process?

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