a Given the following restriction map of a cloned 10 kb piece of DNA, what size fragments would you see after digesting this linear DNA fragment with each of the enzymes or combinations of enzymes listed: (1) EcoRI, (2) BamHI, (3) EcoRI + HindlII, (4) BamHI + Psl, and (5) EcoRI + BamHI. b. What fragments in the last three double digests would hybridize with a probe made from the 4 kb BamHI fragment? HH E B E 15 06 10 1.2 2.1 1.9 1.7 kb

Clone number in this case is number 196 which is shown in the images.  State whether a BamHI site has been re-created at the forward- and the reverse-end junctions of the human DNA with the plasmid vector  band sizes are shown in one of the images. (0.5, 1, 2, 3, 4, 5, 6, 8, 10kb)

After Drosophila DNA has been treated with a restrictionenzyme, the fragments are inserted into plasmids and selected as clones in E. coli. With the use of this “shotgun”technique, every DNA sequence of Drosophila in a librarycan be recovered.a. How would you identify a clone that contains DNAencoding the protein actin, whose amino acid sequenceis known?b. How would you identify a clone encoding a specifictRNA

24. Sickle-cell anemia arises from a mutation in the gene for the B chain of human hemoglobin. The change from GAG to GTG in the mutant that is located within the cleavage site for the restriction enzyme Mstll, which recognizes the target sequence CCTGAGG. These findings form the basis of a diagnostic test for the sickle-cell gene. Which answer best explains how this test should work? A) In combination with DNA polymerase, application of Mstll will produce long DNA strands with the normal gene but not the mutant gene. B) In combination with DNA polymerase, application of Mstll will produce long DNA strands with the mutant gene but not the normal gene. C) Treatment with Mstll produces DNA fragments from the normal gene but cannot cleave the normal gene. D) Treatment with Mstll produces DNA fragments from the normal gene but cannot cleave the mutant gene. E) Restriction enzymes are non-specific relative to the binding requirements for such a test. 25. The restriction enzymes Kpnl and…