a) Examine the nudeotide sequence below, and determine the amino acid sequence encoded by this mRNA. (2) 5 CCUCCGGACCGGAUGCCCGCGGCAGCUGCUGAACCAUGGCCCGCGGGUGAGCCAAGGAGGAGGGC 3' b) What would be the consequence of a mutation that resulted in changing the underlined nucleotide to a G? (2)

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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a)
Examine the nucleotide sequence below, and determine the amino acid sequence encoded by this mRNA. (2)
5' CCUCCGGACCGGAUGCCCGCGGCAGCUGCUGAACCAUGGCCCGCGGGUGAGCCAAGGAGGAGGGC 3'
b)
What would be the consequence of a mutation that resulted in changing the underlined nucleotide to a G? (2)
Second base
U
G.
Consensus sequences functioning in transcription or translation (5-3):
UGU
UAU
UCU
Phe
UCC
Ser
UCA
Leu
UCG
UUU
Tyr
Cys
TATA box (-25)
TATAAA
UUC
UAC
UGC
UAA Stop UGA Stop A
UAG Stop UGG Trp G
UUA
TFIIB recognition element
/c/c/¢CGCC
UUG
TATAAT
CGU
CAU
His
CAC
Pro
CAA
Gln
CAG
-10 (Pribnow) sequence
CUU
CCU
CC
Leu
CCA
CGC
Arg
CGA
CUC
TTGACA
-35 sequence
CỦA
CUG
CCG
CGG
Shine-Dalgarno sequence (Ribosome binding site) UAAGGAGGU
YYANT/AYY
AGU
Asn
AGC
AUU
ACU
AAU
Ser
Initiator element
AUC lle ACC
Thr
AAC
AGA
Lys
AGG
AUA
ACA
AAA
lA AGLGU ^/G AGU
Arg
Intron 5' splice site
AUG Met ACG
AAG
CAGIG
GGU
GAU
Asp
GAC
Intron 3' splice site
GCU
GUU
GCC
Val
GCA
GGC
Gly
GGA
GUC
AAUAAA
Ala
Cleavage site
GAA
Glu
GAG
GUA
A/G CCAUGG
GUG
GCG
GGG
Kozak sequence
Third base
UCAG
UCAG
UCAG
UCAG
First base
Transcribed Image Text:a) Examine the nucleotide sequence below, and determine the amino acid sequence encoded by this mRNA. (2) 5' CCUCCGGACCGGAUGCCCGCGGCAGCUGCUGAACCAUGGCCCGCGGGUGAGCCAAGGAGGAGGGC 3' b) What would be the consequence of a mutation that resulted in changing the underlined nucleotide to a G? (2) Second base U G. Consensus sequences functioning in transcription or translation (5-3): UGU UAU UCU Phe UCC Ser UCA Leu UCG UUU Tyr Cys TATA box (-25) TATAAA UUC UAC UGC UAA Stop UGA Stop A UAG Stop UGG Trp G UUA TFIIB recognition element /c/c/¢CGCC UUG TATAAT CGU CAU His CAC Pro CAA Gln CAG -10 (Pribnow) sequence CUU CCU CC Leu CCA CGC Arg CGA CUC TTGACA -35 sequence CỦA CUG CCG CGG Shine-Dalgarno sequence (Ribosome binding site) UAAGGAGGU YYANT/AYY AGU Asn AGC AUU ACU AAU Ser Initiator element AUC lle ACC Thr AAC AGA Lys AGG AUA ACA AAA lA AGLGU ^/G AGU Arg Intron 5' splice site AUG Met ACG AAG CAGIG GGU GAU Asp GAC Intron 3' splice site GCU GUU GCC Val GCA GGC Gly GGA GUC AAUAAA Ala Cleavage site GAA Glu GAG GUA A/G CCAUGG GUG GCG GGG Kozak sequence Third base UCAG UCAG UCAG UCAG First base
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