need to prepare a standard calibration curve for gamma globulin. absorbances on Y and mg of standard protein per assay on X. used 0.1mg/ml gamma stock for tubes 2-6. (Water (ml), gamma (ml), Abosrbance)--> (.036, .004, .290) (.036, .008, .358) (.024, .016, .341) (.016, .024, .520) (.008, .032, .597) - What is the math and how do you get the standard curve?

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KDa 97.4 66.24 45.0 31.0 21.5 14.4 a For each condition (there are 5 different conditions), is there a higher percentage of the total protein in the supernatant, pellet, or is it about equal? For condition 1 - For condition 2 - For condition 3 - For condition 4 - For condition 5 -

A physician orders a 375 mg/m2 dose of Rituxan in 500 mL NS for a patient with a BSA of . Rituxan is available in 10 mL vials in a concentration of 10 mg/mL. How many mL will be added to the IV bag?

What would the order of elution be (first to last to come out of a size-exclusion chromatography column) for the mixture of myoglobin (17.7 kDa), hemoglobin (64.5 kDa), lysozyme (14.3 kDa) & albumin (67 kDa)? a. Albumin, hemoglobin, lysozyme, myoglobin Ob. Hemoglobin, myoglobin, lysozyme, albumin Oc. Lysozyme, myoglobin, albumin, hemoglobin Od. Albumin, hemoglobin, myoglobin, lysozyme

Bicillin CR is given IM. There are 600,000 units/mL in each syringe and the dose is 1.5 million units (1,500,000 units). What volume is to be administered?

A 10-week-old child suffered from a salt-wasting crisis. His adrenal glands were enlarged and other organs were normal size. The child is phenotypically female, karotyping showed that the child is 46XY. The biochemical profile shows as: - sodium of 109 (reference range: 134-147) - potassium of 6.1 (reference range: 3.5-5.0) - pregnenlone of 0.002 (reference range: 0.3-1.5) - renin activity of 38 (reference range: 0.5-3.5) - 17-OH pregnenolone of 0.01 (reference range: 6.8-93.5) - ACTH of 1960 (reference range: 0.5-3.5) The cholesterol esters in the adrenal glands accumulated and the concentration of ∆4-androstenedione was not detectable. Also, the enzymes in the steroid biosynthesis did not have detrimental mutations. The child was given treatment with glucocorticoid and mineralocorticoid. Question: a) Which protein is deficient in this case and explain the rationale.

DILUTION COLONY COUNT CFU/mL 1:106 155000   1:107 15500   1:108 1550   1:109 155   Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?

When 300 mL of 0.0010 M Ca( NO3)2 solution is added exactly to 500 mL of 0.0015 M K3PO4 solution, find the ion product Q and decide whether to precipitate. (However, K_sp of Ca3(PO4)2 = 1.2x 10^-26)

Please don't provide handwritten solution ....  In sickle cell anemia, only one substitution of glutamic acid by valine (one amino acid substitution), is known to be the most possible cause that changes normal red blood cells into sickle cell red blood cells. True False