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STEM Workplace Practices Q9
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- 1. What happens if you try to use the coarse adjustment when the10Xlens is in place?4) Define Sparse Coding. 5) What is the purpose of the Faraday cage?4. Study the microscope drawing on page 11 and the description of the parts on the opposite page and then answer these questions. [a]What is the total magnification when the 4ox objective lens is being used? [b]Which objective lens "sees" the largest area of the slide? [c]Which focusing knob is used at high power? [d]What devise is used to change the amount of light coming through the object? devices are aná
- 7. The spectrophotometer is used to measure absorbance experimentally. Briefly, explain how this is accomplished.1. What are the three properties of laser light that are different than normallight? 2. How is distention achieved in minimally invasive procedures, and why isit necessary?17. What can happen if you only use one eye to look into a binocular microscope? 18. Why do you only use the Fine Adjustment Knob to focus at high magnifications? 19. Why do you only add a single drop of water to a specimen when creating a wet mount?
- 5. In lab, you learned that magnification and field diameter are inversely related to each other. If 40X total magnification has a field diameter of 4.5 mm and 100X total magnification has a field diameter of 1.8 mm, what is the field diameter of the total magnification of 1000X in a compound microscope? Show your work. (Continued from question 4). Are you able to obtain this value from using the vernier system on the microscope? Explain.4. If the diameter of the field of view (FOV) for your microscope is 5.5 mm at 40x total magnification, what is the diameter of the FOV at 1000X? Show work and don't forget units! 5. Use the counting method to estimate the length of an elodea cell. You will need to use your calculation for the FOV at 400x total magnification. Show work and don't forget units! Elodea Leaf at 400x total mag 6. Use the percent method to estimate the size of a human cheek epithelial cell. You will need to use your calculation for the FOV at 1000x total magnification. Show work and don't forget units! Cell Membrane Nucle us1. What happens to your image if you try to magnify it using 40x or 100x?
- 1. In microscopy, what could be the possible reason why we cannot completely resolve the specimen under the Oil Immersion Objective (OIO) when, in fact, it was taught that the OIO has a resolving poewer? 2. When using the OIO, we can only focus on one group or parts at a time. (that is, the other parts are blurred) Whys is it so?8. Objective lens X has a limit of resolution equal to 0.2 mm while objective lens Y has 30 pm. a. Which among the two can magnify a mosquito wing in finer detail? b. Which among the two would show a greater area of the mosquito wing?3. An object is 20µm in diameter. If viewed through a 10X ocular lens and a 40X objective lens, how large will the object appear (what are the magnified dimensions of the object)?