12. Shown below are three possible mechanisms for DNA replication. Identify which each kind is (semiconservative, conservative, or dispersive). I Which model is this? (select one) A. Semiconservative Conservative Dispersive Semiconservative Conservative Dispersive Semiconservative Conservative Dispersive B. C. XXX< YOX XXXXX xxxxx XXXXXXX xxx<1000x XXXXXX xx

On paper, replicate the following segment of DNA:            5’ A T C G G C T A C G T T C A C 3’            3’ T A G C C G A T G C A A G T G 5’a. Show the direction of replication of the new strands and explainwhat causes lagging and leading strands to show different patternsof replication.b. Explain how this is semiconservative replication. Are the newstrands identical to the original segment of DNA?

4) Replication of a circular DNA molecule can occur by either theta replication or by rolling circle replication. Describe or explain three differences between these two modes of DNA replication (you must accurately describe the differences between the two - an accurate statement about one form of replication that does not clearly indicate how the other form differs will not answer the qestion in it's entirety).

#3 under Identify the Structures is what?   A. phosphate polymerase complex   B. DNA polymerase complex   C. deoxyribose sugar polymerase complex   D. nitrogen base polymerase complex

33.Choose the DNA nucleotide sequence that would be complementary to the following strand (note: pay attention to DIRECTION of replication): 5' GTAGTCA 3' a 3' UATUAGA 5' b. 5' TGACTAC 3' 3' ACGACTG 5' 5' UGACUAC 3'. 5.Using agarose gel electrophoresis to analyze DNA, short DNA travels the long DNA. a faster than slower than at the same speed as d. Question 6 Which type of bonds link the individual nucleotides together in DNA? Glycosidic bond Peptide bond Phosphodiester bond Electrostatic bond a e Hydrogen bond Question 7 A typical engineered plasmid vector often contains the following features except a A poly linker region Origin of replication A signal peptide An antibiotic resistant gene a. b. C. C. d.

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1. Okazaki fragments are small pieces of DNA synthesized discontinuously on the lagging strand of DNA during replication (true or false) 2. during replication DNA polymerase synthesize DNA in the 5 or 3 direction (true or false)

DNA methyltransferases are important regulators of epigenetic marks/memory. What is the role of a DNA methyltransferase during DNA replication? A. They catalyze the addition of methyl groups to the new daughter strand using the parental strand's methylation pattern B. They remove all methyl tags from all of the DNA restoring the totipotent state prior to mitosis C. They catalyze the transfer of acetyl groups to certain nucleotides within the DNA after DNA replication has been completed D. They inhibit the methylation of the new daughter strand so that only one strand remains methylated after replication

18. If 32P is used to label both strands of double-stranded DNA, after two rounds of replication, four double-stranded molecules of DNA are produced. Of these,a. one of the four double-stranded molecules of DNA will contain all the 32Pb. two of the four double-stranded molecules of DNA will contain 32P, each with half of the 32Pc. three of the four double-stranded molecules of DNA will contain 32P, each with 1/3 of the 32Pd. all four double-stranded molecules of DNA will contain 32P, each with ¼ of the 32Pe. none of the double-stranded molecules of DNA will contain 32P

1. Name all the proteins that are in the DNA replication fork in E. Coli, and describe the functions of these proteins. Explain how two DNA strands are replicated at the same time by one replication fork.