4.46. Mammalian cells can be cultured for a variety of purposes, including synthesis of vaccines. They must be maintained in growth media containing all of the components required for proper cellular function to ensure their survival and propagation. Traditionally, growth media were prepared by blending a powder, such as Dulbecco's Modified Eagle Medium (DMEM) with sterile deionized water. DMEM contains glucose, buffering agents, proteins, and amino acids. Using a sterile (i.e., bacterial-, fungal-, Adapted from a problem contributed by Adam Melvin of Louisiana State University. Problems 191 and yeast-free) growth medium ensures proper cell growth, but sometimes the water (or powder) can become contaminated, requiring the addition of antibiotics to eliminate undesired contaminants. The culture medium is supplemented with fetal bovine serum (FBS) that contains additional growth factors required by the cells. Suppose an aqueous stream (SG = 0.90) contaminated with bacteria is split, with 75% being fed to a mixing unit to dissolve a powdered mixture of DMEM contaminated with the same bacteria found in the water. The ratio of impure feed water to powder entering the mixer is 4.4:1. The stream leaving the mixer (containing DMEM, water, and bacteria) is combined with the remaining 25% of the aqueous stream and fed to a filtration unit to remove all of the bacteria that have contaminated the system, a total of 20.0 kg. Once the bacteria have been removed, the sterile medium is combined with FBS and the antibiotic cocktail PSG (Penicillin-Streptomycin-L-Glutamine) in a shaking unit to generate 5000 L of growth medium (SG = 1.2). The final composition of the growth medium is 66.0 wt% H₂O, 11.0% FBS, 8.0% PSG, and the balance DMEM. (a) Draw and label the process flowchart. (b) Do a degree-of-freedom analysis around each piece of equipment (mixer, filter, and shaker), the splitter, the mixing point, and the overall system. Based on the analysis, identify which system or piece of equipment should be the starting point for further calculations. (e) Calculate all of the unknown process variables. (d) Determine a value for (i) the mass ratio of sterile growth medium product to feed water and (ii) the mass ratio of bacteria in the water to bacteria in the powder. (e) Suggest two reasons why the bacteria should be removed from the system.
4.46. Mammalian cells can be cultured for a variety of purposes, including synthesis of vaccines. They must be maintained in growth media containing all of the components required for proper cellular function to ensure their survival and propagation. Traditionally, growth media were prepared by blending a powder, such as Dulbecco's Modified Eagle Medium (DMEM) with sterile deionized water. DMEM contains glucose, buffering agents, proteins, and amino acids. Using a sterile (i.e., bacterial-, fungal-, Adapted from a problem contributed by Adam Melvin of Louisiana State University. Problems 191 and yeast-free) growth medium ensures proper cell growth, but sometimes the water (or powder) can become contaminated, requiring the addition of antibiotics to eliminate undesired contaminants. The culture medium is supplemented with fetal bovine serum (FBS) that contains additional growth factors required by the cells. Suppose an aqueous stream (SG = 0.90) contaminated with bacteria is split, with 75% being fed to a mixing unit to dissolve a powdered mixture of DMEM contaminated with the same bacteria found in the water. The ratio of impure feed water to powder entering the mixer is 4.4:1. The stream leaving the mixer (containing DMEM, water, and bacteria) is combined with the remaining 25% of the aqueous stream and fed to a filtration unit to remove all of the bacteria that have contaminated the system, a total of 20.0 kg. Once the bacteria have been removed, the sterile medium is combined with FBS and the antibiotic cocktail PSG (Penicillin-Streptomycin-L-Glutamine) in a shaking unit to generate 5000 L of growth medium (SG = 1.2). The final composition of the growth medium is 66.0 wt% H₂O, 11.0% FBS, 8.0% PSG, and the balance DMEM. (a) Draw and label the process flowchart. (b) Do a degree-of-freedom analysis around each piece of equipment (mixer, filter, and shaker), the splitter, the mixing point, and the overall system. Based on the analysis, identify which system or piece of equipment should be the starting point for further calculations. (e) Calculate all of the unknown process variables. (d) Determine a value for (i) the mass ratio of sterile growth medium product to feed water and (ii) the mass ratio of bacteria in the water to bacteria in the powder. (e) Suggest two reasons why the bacteria should be removed from the system.
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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Transcribed Image Text:*4.46. Mammalian cells can be cultured for a variety of purposes, including synthesis of vaccines. They must
be maintained in growth media containing all of the components required for proper cellular function to
ensure their survival and propagation. Traditionally, growth media were prepared by blending a
powder, such as Dulbecco's Modified Eagle Medium (DMEM) with sterile deionized water. DMEM
contains glucose, buffering agents, proteins, and amino acids. Using a sterile (i.e., bacterial-, fungal-,
*Adapted from a problem contributed by Adam Melvin of Louisiana State University.
Problems 191
and yeast-free) growth medium ensures proper cell growth, but sometimes the water (or powder) can
become contaminated, requiring the addition of antibiotics to eliminate undesired contaminants. The
culture medium is supplemented with fetal bovine serum (FBS) that contains additional growth factors
required by the cells.
Suppose an aqueous stream (SG = 0.90) contaminated with bacteria is split, with 75% being fed to
a mixing unit to dissolve a powdered mixture of DMEM contaminated with the same bacteria found in
the water. The ratio of impure feed water to powder entering the mixer is 4.4:1. The stream leaving the
mixer (containing DMEM, water, and bacteria) is combined with the remaining 25% of the aqueous
stream and fed to a filtration unit to remove all of the bacteria that have contaminated the system, a total
of 20.0 kg. Once the bacteria have been removed, the sterile medium is combined with FBS and the
antibiotic cocktail PSG (Penicillin-Streptomycin-L-Glutamine) in a shaking unit to generate 5000 L of
growth medium (SG = 1.2). The final composition of the growth medium is 66.0 wt% H₂O, 11.0%
FBS, 8.0% PSG, and the balance DMEM.
(a) Draw and label the process flowchart.
(b) Do a degree-of-freedom analysis around each piece of equipment (mixer, filter, and shaker), the
splitter, the mixing point, and the overall system. Based on the analysis, identify which system or
piece of equipment should be the starting point for further calculations.
(c) Calculate all of the unknown process variables.
(d) Determine a value for (i) the mass ratio of sterile growth medium product to feed water and (ii) the
mass ratio of bacteria in the water to bacteria in the powder.
(e) Suggest two reasons why the bacteria should be removed from the system.
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