Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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- Additional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain, Flagella stain. What is a positive and negative results of this procedures?arrow_forwardIdentify the cellular morphology for this Gram stain. L! What is the Gram reaction? What is the bacterial shape? What is the bacterial arrangement? in What is the staining dye? [Choose ] [Choose ] Phenol Red Gram-negative Singles no arrangement Safranin Gram-positive Cocci Bacilli Crystal Violet Chains Clusters Spirillaarrow_forwardizzes/00071/take/questions/600381 Regarding Gram staining, what will be the appearance of the Gram-positive bacteria if... All steps are done correctly? The slide is not heat-fixed prior to staining? Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? I Not enough acetone:alcohol was used? Carbol fuschin or safranin was omitted? W [Choose ] [Choose ] Purple Clear Pink Orange [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] 88 22°Carrow_forward
- 5) Which is the most crucial step in the Gram staining procedure? Why?arrow_forwardOut of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis). Which of the following test(s) will help me identify the unknown gram-positive bacteria? Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Testarrow_forwardWhat is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handlearrow_forward
- Identify the cellular morphology for this Gram stain. What is the Gram reaction? What is the bacterial shape? What is the bacterial arrangement? What is the staining dye? [Choose ] [Choose ] Cocci Singles no arrangement Pleomorphic Chains Gram-negative Crystal Violet Phenol Red Bacilli Gram-positive Clusters Safraninarrow_forwarda. Identify the cell shape, cell arrangement, and Gram reaction of the Unknown microbe in the image below. b. Suppose the corresponding Gram negative control were purple. Explain what step(s) of the Gram stain you would adjust and how you would adjust them to ensure the accuracy of your Unknown resultsarrow_forwardList the primary stain, secondary stain, mordant, and decolorizer for the gram stain, and write them in the order that you would use them. Make sure to label which is which.arrow_forward
- After performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explain why, the result you would want to observe and the interpretation of the result.arrow_forwardExplain how you would troubleshoot a Gram stain of a known G(-) organism that stained purple.arrow_forwardDecolorization in Gram-staining removes safranin from gram-negative cells True Falsearrow_forward
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