Given an 8 M potassium chloride stock solution, explain whether it is possible to perform a dilution resulting in an 11 M working solution.
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Given an 8 M potassium chloride stock solution, explain whether it is possible to perform a dilution resulting in an 11 M working solution.
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- give the respective the TOTAL DILUTION FACTOR of respective serial dilutions. show complete solution.If a 250 ml solution of ethanol in water is prepared with 4 ml of absolute ethanol (100%), what is the dilution factor?To make 100 ml 0.3% NP-40 solution for FISH post washing, how many ul 100% NP-40 solution is needed?
- A pharmacist prepares 80 mL of an isotonic solution of 1.1 %w/v Lidocaine HCl (E-value 0.2). To prepare the solution, he dissolves Lidocaine HCl in purified water to make an isotonic solution. Then he dilutes the Lidocaine HCl solution with 0.9%w/v sterile sodium chloride to complete the formulation. How many milliliters of purified water is needed to make the formulation by this method?Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?An aqueous solution of 0.2100 g of a mixture containing potassium cyanide and potassium chloride required 14.56 mL of 0.1000 M silver nitrate to produce a faint permanent turbidity; 30.00 mL more of the silver nitrate was added (an excess) and the precipitate of AgCl and AgCN filtered off from the solution. The filtrate and washings were titrated with 13.06 mL 0.1000 M thiocyanate solution. Calculate the percentages of KCl and KCN in the sample. Note: the appearance of faint turbidity indicates the formation of Ag(CN)2-
- a)Determine the amount of X (in gram) in order to prepare 100 mL of 50 mM stock solution of X. Show your work. (MW of X= 225) b)Determine the volume required (mL) from the stock solution X in (i) to prepare 500 mL of MS medium containing 5 mg/L X. Show your work.How do you prepare a 100mL of 0.1 M phosphate buffer? From the anterior buffer, how do you make 100mL of 0.05 M? PLEASE GIVE THE MEASUREMENTS3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mL
- A 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?How much of a 10x buffer should be added to 900ml of water to make a 1x solution?Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.