14. A student makes a smear by applying a bacteria onto the slide. A simple stain is performed, but when the student looks under the microscope, the organisms are misshapen and look weird. What did the student do wrong when making the smear?

Put the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it.  Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersion

Fill in the blank: The gram stain is a ______________ stain in which a decolorization step occurs between the application of two basic stains.

How can the thickness of the smear affect the staining procedure?