1. You are studying an E. coli gene that specifies a protein. A part of its sequence is -Ala-Pro-Trp-Ser-Glu-Lys-Cys-His You recover two mutants for this gene that show no enzymatic activity. By isolating the mutant enzyme products, you find the following sequences: Mutant 1: Ala-Pro-Trp-Arg-Glu-Lys-Cys-His Mutant 2: Ala-Pro- What is the molecular basis for mutation 1 and for mutation 2? What is the DNA sequence that specifies this part of the protein? Wild-type amino acid sequence: NH3-Ala-Pro-Trp-Ser-Glu-Lys-Cys-His-COOH Mutant 1: NH3-Ala-Pro-Trp-Arg-Glu-Lys-Cys-His-COOH Mutant 2: NH3-Ala-Pro-

Q: If the following truncated peptide represents the active domain of the COVID19’s S-protein…

A: Mutations are alterations in the DNA sequence of an organism. Small changes, such as adding or…

Q: A protein substrate is known to be multi-monoubiquitinated. The control lane "C" in the SDS-PAGE gel…

A: The proteins are separated on the basis of their molecular weight by the technique known as Sodium…

Q: 2) Myoclonal epilepsy and ragged red fiber disease (MERRF) is a human condition named for the ragged…

A: Mitochondrial tRNA in humans has gained interest due to the discovery of associations between point…

Q: 14. Which mutation would be more harmful to a cell? For each predict the outcome of each mutation…

A: In order to base-pair with the correct codon on the mRNA during protein synthesis, the anticodon is…

Q: D D Intron +1 E E A В В C GGGCGG GCCCAATCT TATAAA Intron -5000 -100 -25 1. What region functions…

A: The transcription is the process of conversion DNA to RNA . DNA stands for dioxy ribonucleic acid…

Q: 1 0.8 0.6 Mutant y 0.4 Wild-Type 0.2 40 60 80 [ligand] 20

A: According to the given information, the wild type protein structure has aspartate in its primary…

Q: An RNA polymer is made by using the enzyme polynucleotide phosphorylase with equal quantities of CTP…

A: Answer : glycine, proline, alanine, arginine,  The possible codons can be  CCG CGC GCC GGC GCG CGG

Q: 1. Certain proteins that stimulate expression of a gene bind to DNA in a sequence specific manner…

A: Proteins are an important organic compound. Some proteins stimulate the expression of a gene bind to…

Q: mitochondrial genes affecting tRNAs. For example, one form of MELAS (mitochondrial myopathy,…

A: Mitochondrial diseases ( MELAS) are a heterogeneous group of rare genetic disease  it is caused…

Q: 15. Cellular proteins are oftentimes post-translationally modified. Choose one of the following…

A: Post translational modification are the modifications done in the protein by covalently attaching…

Q: 2) Myoclonal epilepsy and ragged red fiber disease (MERRF) is a human condition named for the ragged…

A: Myoclonic epilepsy:It is related to a family of epilepsies which are present along with myoclonus.…

Q: (a) () The isoelectric point (pl) of the protein, i.e., the pH at which the overall electrostatic…

A: The isoelectric point (pI) is the pH of a solution at which a given protein has a net charge of…

Q: You are interested in studying a unique protein found in a rare wildflower found in the desert…

A: The objective of the question is to understand the changes brought about by a mutation in a protein…

Q: Christian Anfinsen showed that the enzyme Ribonuclease (RNase) is completely inactivated at high…

A: In the 1950s, Christian Anfinsen performed a series of studies. He discovered that all the…

Q: A poison added to an in vitro translation mixture containing mRNA molecules with the sequence 5'-…

A: In the process of protein synthesis, when the ribosome encounters the start codon (AUG) and…

Q: The following RNA sequence represents a small messenger which can be translated in a prokaryotic…

A: The first step in translating an RNA sequence into a protein is to divide the sequence into codons,…

Q: Consider the following coding 71 nucleotide DNA template sequence (It does not contain a…

A: In cell protein formed according to the sequence on mRNA. mRNA is formed from DNA sequence by the…

Q: In bacteria, researchers have isolated strains that carry mutations within tRNA genes. These…

A: Codon is located on mRNA while anticodon is on tRNA. Anticodons specifically identifies and pair to…

Q: What is the primary difference betwee class I and class II aminoacyl-tRNA synthetases.   a. Class…

A: An enzyme that synthesizes tRNAs by amino acid polymerization is called an aminoacyl-tRNA synthetase…

Q: The antibiotic paromomycin binds to a ribosome and induces the same conformational changes in 16S…

A: Ribosomes are complex molecules that are composed of ribosomal RNA molecules where photosynthesis…

Q: In the: Inhibition of splicing by ribozymes Explain: (a) What is the process affected? (b) What is…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: 28. a. Can a tRNA exist that has the anticodon sequence 5' IAA? If so, which amino acid would it…

A: Transfer RNA (tRNA) molecules contain a sequence of three nucleotides known as the anticodon. It is…

Q: Describe the two sequential reactions that occur in the activesite of aminoacyl-tRNA synthetases.

A: This two-step reaction of aminoacyl-tRNA synthetases occurs during the process of protein synthesis.

Q: A mutation that changes an alanine to a leucine in the nucleus of a protein leads to a less active…

A: Introduction: Each organism's DNA sequence is unique. Its base-pairs sequence can change from time…

Q: N-terminal region variable sequence C-terminal region of

A: Introduction: Cytosolic N terminal region meant it is extended towards the cytosol. The membrane…

Q: Select one: O Met-Cys-Ala-Thr-Tyr---- O Met-Cys-Cys-Thr-Tyr--- O Met-Ala-Ala-Thr-Tyr---- O…

A: Aminoacyl tRNA synthetase are enzymes of extreme importance during the process of protein synthesis…

Q: The concentration of RNase that Christian Anfinsen used in his denaturation/renaturation experiments…

A: Anfinsen's work demonstrated that the native structure of ribonuclease will form after denaturation…

Q: 7. You want to study the effect of lysine methylation on gene regulation. The lysine amino acid…

A: Enzymes are known as biological catalysts, which accelerate the rate of the reaction without…

Q: An E. coli lac- mutant (a mutant that cannot grow in the medium containing lactose as only carbon…

A: Operon, is a bacterial and viral genetic regulation system in which genes coding for functionally…

Q: What potential polypeptides can be produced from the following mRNA sequence? There are more than…

A: In our body genetic information is stored in form of DNA. DNA multiples itself by replication. DNA…

Q: 5' cap amino acid aminoacyl tRNA synthetase anticodon codon EF-Tu-GTP EF-Ts eukaryote Kozak…

A: Three process combined together to form a central dogma that is replication where copy of genetic…

Q: One single polypeptide chain  (120 amino acid residues) is produced for protein A in prokaryotic…

A: A polypeptide chain is a linear or cyclic  compounds which have amino acids as the building units of…

Q: The following enzyme has an exact integer number of turns. What is the length and amount of amino…

A: Enzyme are usually composed of proteins while some enzymes are ribozymes (RNA enzymes). Proteins are…

Q: 7). You analyze a biosynthetic pathway in an unknown strain of bacteria. Given the following data…

A: 3-phosphoglycerate (PGA) is used by bacteria as the starting material to synthesize serine. From…

Q: . Seven E. coli mutants were isolated. The activity ofthe enzyme β-galactosidase produced by cells…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: 1 An Amino Acid Sequence Leu - Tyr - Gly-Gly - Val Which of the following changes to the mRNA that…

A: The amino acids in the given sequence are leucine, tyrosine, glycine, glycine, and valine.

Q: 2) A group of three researchers (who often tend to disagree with one another) isolated a new protein…

A: Protein: It is a macromolecule that is made up of amino acids joined together with a Polypeptide…

Q: a- Name the figure which shows the Co-translational translocation. A or B b -list the molecules…

A: The protein that are destined for secretion, the membrane proteins and the lysosomal proteins are…

Q: 8) A Leu > Ala mutation at a site buried in the core of the enzyme lysozyme ends up being…

A: Most globular proteins include significant quantities of α-helix and β-strand. Globular proteins can…

Q: The following polynucleotide was synthesized and used as a template forpeptide synthesis in a…

A: Three consecutive nucleotides form a triplet termed a codon that codes for a single amino acid.…

Q: HbS results from the substitution of valine forglutamic acid at the number 6 position in the b…

A: HbS is the result of a single base-pair mutation in the gene responsible for the beta-globin chain…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.