1. Various types of vesicle coats have been implicated in membrane traffic pathways such as endocytosis, export from the ER to the Golgi, and transport between Golgi cisternae. But so far, no coat has been identified for the secretory vesicles that carry material from the Golgi to the plasma membrane. Do you think such a coat is likely to exist? Justify your answer.

Biology 2e
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ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter4: Cell Structure
Section: Chapter Questions
Problem 20RQ: Which of the following do not play a role in intracellular movement? microfilaments and intermediate...
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Evidence Question #7
1. Various types of vesicle coats have been implicated in membrane traffic pathways such
as endocytosis, export from the ER to the Golgi, and transport between Golgi
cisternae. But so far, no coat has been identified for the secretory vesicles that carry
material from the Golgi to the plasma membrane. Do you think such a coat is likely to
exist? Justify your answer.
2. A muscle cell contains acetylcholine receptors that act as ligand-gated cation channels,
and it also contains voltage-gated Na+ channels of the type found in neurons. In
theory, could a muscle cell get away with having only acetylcholine-gated cation
channels, which would serve the dual purpose of receiving neurotransmitter signals
and propagating action potentials? Why or why not?
3. Fluorescent FM dyes partition reversibly into biological membranes without
penetrating through them. Suppose that you have neurons cultured in a dish. You
incubate the neurons with an FM dye, and then you wash them with medium to
remove the dye.
a) You find that if the neurons are stimulated to trigger action potentials during
incubation with the dye, the synaptic regions of the neurons remain fluorescent
after the wash. Propose an explanation for this effect.
b) After generating fluorescent synaptic regions by this procedure, suppose that you
trigger additional action potentials while continuing to wash with medium. Would
you expect the synaptic regions to lose fluorescence? Explain.
Transcribed Image Text:Evidence Question #7 1. Various types of vesicle coats have been implicated in membrane traffic pathways such as endocytosis, export from the ER to the Golgi, and transport between Golgi cisternae. But so far, no coat has been identified for the secretory vesicles that carry material from the Golgi to the plasma membrane. Do you think such a coat is likely to exist? Justify your answer. 2. A muscle cell contains acetylcholine receptors that act as ligand-gated cation channels, and it also contains voltage-gated Na+ channels of the type found in neurons. In theory, could a muscle cell get away with having only acetylcholine-gated cation channels, which would serve the dual purpose of receiving neurotransmitter signals and propagating action potentials? Why or why not? 3. Fluorescent FM dyes partition reversibly into biological membranes without penetrating through them. Suppose that you have neurons cultured in a dish. You incubate the neurons with an FM dye, and then you wash them with medium to remove the dye. a) You find that if the neurons are stimulated to trigger action potentials during incubation with the dye, the synaptic regions of the neurons remain fluorescent after the wash. Propose an explanation for this effect. b) After generating fluorescent synaptic regions by this procedure, suppose that you trigger additional action potentials while continuing to wash with medium. Would you expect the synaptic regions to lose fluorescence? Explain.
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