A. Dr. Randy Schekman introduced you to a yeast model system for studying membrane fusion and vesicle trafficking. Imagine that you have isolated a new temperature-sensitive sec mutant which you suspect has a specific defect in secretory vesicle docking and fusion with the plasma membrane (exocytosis). Describe an experiment you could do to test your hypothesis. B. Now, explain how you would continue your experiment to identify which secretory pathway proteins are present on these secretory vesicles. C. Finally, among the proteins present in these synaptic vesicles according to mass spec you find a protein which contains a G-domain fold suggesting that it is a G-protein. Briefly outline how, in principle, you would determine which of the other proteins is a GAP for this G protein.
A. Dr. Randy Schekman introduced you to a yeast model system for studying membrane fusion and vesicle trafficking. Imagine that you have isolated a new temperature-sensitive sec mutant which you suspect has a specific defect in secretory vesicle docking and fusion with the plasma membrane (exocytosis). Describe an experiment you could do to test your hypothesis. B. Now, explain how you would continue your experiment to identify which secretory pathway proteins are present on these secretory vesicles. C. Finally, among the proteins present in these synaptic vesicles according to mass spec you find a protein which contains a G-domain fold suggesting that it is a G-protein. Briefly outline how, in principle, you would determine which of the other proteins is a GAP for this G protein.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
A. Dr. Randy Schekman introduced you to a yeast model system for studying membrane fusion and vesicle trafficking. Imagine that you have isolated a new temperature-sensitive sec mutant which you suspect has a specific defect in secretory vesicle docking and fusion with the plasma membrane (exocytosis). Describe an experiment you could do to test your hypothesis.
B. Now, explain how you would continue your experiment to identify which secretory pathway proteins are present on these secretory vesicles.
C. Finally, among the proteins present in these synaptic vesicles according to mass spec you find a protein which contains a G-domain fold suggesting that it is a G-protein. Briefly outline how, in principle, you would determine which of the other proteins is a GAP for this G protein.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 3 steps
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON