1. Using the following chromatogram, perform the calculations requested. Include the equation used and any work necessary to obtain your answer Response → A B (to = 1.0 min.). Time → tR (min) 2.45 5.55 Wn (min) 0.06 0.09 a. What is KA? b. What is NB? c. What is Rs (Using NB for the efficiency term)? d. What is a? e. What is Rs if the length of the column is doubled? f. What happens to a when L is doubled?

10 7 Ruler is in 6 cm unit 5 A B 3 Standards Sample from reaction mixture att = 30 mins Sample from reaction mixture att = 60 mins a. Which type of chromatography based on the polarity of the stationary phase was employed in the experiment? b. Identify the limiting and excess reactants. c. Which product appeared after 30 minutes? d. Which product was produced only after 60 minutes? e. Calculate the retention factor of each reactant and product.

-If ethanol (S.G. = 0.790) were used rather than water in measuring the density of the irregular objects, how would that have affected the result? Explain.   -When you were measuring the volume of the irregular solid you were told to stir to eliminate bubbles. If the bubbles were not eliminated, how would that affect the density? Explain.   -Suppose the density of 65.0 mL of some liquid was found to be 0.748 g/mL. What would be the specific gravity of 45.0 mL of this liquid?

28 Report Sheet Separation of the Components of a Mixture D. Determination of Percent Recovery Mass of original sample 10.588 Mass of determined (NH Cl+ NaCl+ SiO2) 1-88 8 Differences in these weights g matter recovered Percent recovery of matter %3D = %00 % g original sample Account for your errors. Combor QUESTIONS 1. Could the separation in this experiment have been done in a different order? For example, if the mixture was first extracted with water and then both the extract and the insoluble residue were heated to dryness, could you determine the amounts of NaCl, NH,Cl, and SiO, originally present? Why or why not? Consult a handbook to answer these questions. 2. How could you separate barium sulfate, BaSO4, from NaCl? 3. How could you separate magnesium chloride, MgCl2, from silver chloride, AgCl? 4. How could you separate tellurium dioxide, TeO2, from SiO,? 5. How could you separate lauric acid from a-naphthol? (See Table 3.1.)