Model 1 Model 1 Idealized Graphs of Enzyme inhibition Competitive Inhibition FEE Inh. slope How does Kapp change? How does Vmax Uncompetitive Inhibition app [5] Inh. b. Uncompetitive Inhibitor Mixed Inhibition [5] 1. Using Model 1 above as a reference, fill in the table (choices: increase, decrease, no change) A competitive inhibitor Mixed inhibitor Inh. [1]=o Click or tap here to enter text. Click or tap here to enter text. change? 2. For each situation in Model 1, consider an inhibitor that is better then the one shown in the graph. Draw the graphs you see in Model 1, then draw a new line that would represent the new, better inhibitor. Paste a photo of your drawing below for: a. Competitive inhibitor An uncompetitive inhibitor Click or tap here to enter text. Click or tap here to enter text. Click or tap here to enter text. Click or tap here to enter text.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question
**Model 1**

**Idealized Graphs of Enzyme Inhibition**

- **Competitive Inhibition:**
  - In the presence of an inhibitor (\[I\]), the slope (1/V\_max) increases, leading to increased K\_m,app, while V\_max,app remains unchanged.

- **Uncompetitive Inhibition:**
  - The inhibitor reduces both V\_max and K\_m,app proportionally, leading to parallel lines.

- **Mixed Inhibition:**
  - The presence of an inhibitor affects both V\_max,app and K\_m,app, altering both the slope and intercept.

1. Using Model 1 as a reference, fill in the table (choices: increase, decrease, no change).

|                       | A competitive inhibitor             | An uncompetitive inhibitor         | Mixed inhibitor                    |
|-----------------------|-------------------------------------|------------------------------------|------------------------------------|
| How does K\_m,app change?   | Click or tap here to enter text. | Click or tap here to enter text.   | Click or tap here to enter text.   |
| How does V\_max,app change? | Click or tap here to enter text. | Click or tap here to enter text.   | Click or tap here to enter text.   |

2. For each situation in Model 1, consider an inhibitor that is better than the one shown in the graph. Draw the graphs you see in Model 1, then draw a new line that would represent the new, better inhibitor. Paste a photo of your drawing below for:
   
   a. Competitive Inhibitor

   ![Graph Placeholder](#)

   b. Uncompetitive Inhibitor
   
   ![Graph Placeholder](#)
Transcribed Image Text:**Model 1** **Idealized Graphs of Enzyme Inhibition** - **Competitive Inhibition:** - In the presence of an inhibitor (\[I\]), the slope (1/V\_max) increases, leading to increased K\_m,app, while V\_max,app remains unchanged. - **Uncompetitive Inhibition:** - The inhibitor reduces both V\_max and K\_m,app proportionally, leading to parallel lines. - **Mixed Inhibition:** - The presence of an inhibitor affects both V\_max,app and K\_m,app, altering both the slope and intercept. 1. Using Model 1 as a reference, fill in the table (choices: increase, decrease, no change). | | A competitive inhibitor | An uncompetitive inhibitor | Mixed inhibitor | |-----------------------|-------------------------------------|------------------------------------|------------------------------------| | How does K\_m,app change? | Click or tap here to enter text. | Click or tap here to enter text. | Click or tap here to enter text. | | How does V\_max,app change? | Click or tap here to enter text. | Click or tap here to enter text. | Click or tap here to enter text. | 2. For each situation in Model 1, consider an inhibitor that is better than the one shown in the graph. Draw the graphs you see in Model 1, then draw a new line that would represent the new, better inhibitor. Paste a photo of your drawing below for: a. Competitive Inhibitor ![Graph Placeholder](#) b. Uncompetitive Inhibitor ![Graph Placeholder](#)
**Module 5 Week 1 Assignment: Enzymes**

This is a group assignment. You must work with a partner. Please be sure to have joined a group in Canvas and then upload one file for the group.

The following kinetic data were obtained for an enzyme in the absence of inhibitor (1) or in the presence of two different inhibitors (2 and 3). The enzyme concentration was the same in all of the experiments. Graph these data as a Lineweaver-Burke plot.

| [S] mM | v (umol/ml * sec) |
|--------|--------------------|
|        | Enzyme (1) | Inhibitor (2) | Inhibitor (3) |
| 1      | 12         | 4.3           | 5.5           |
| 2      | 20         | 8             | 9             |
| 4      | 29         | 14            | 13            |
| 8      | 35         | 21            | 16            |
| 12     | 40         | 26            | 18            |

a. Paste a photo of your Lineweaver-Burke plot here. Plot all three lines on one graph.

b. Using the data, fill in the following table:

|               | Vmax                     | Km                       |
|---------------|--------------------------|--------------------------|
| Enzyme        | Click or tap here to enter text. | Click or tap here to enter text. |
|               | Apparent Vmax            | Apparent Km              |
| Inhibitor 2   | Click or tap here to enter text. | Click or tap here to enter text. |
|               | Type of inhibition       |                          |
|               | Click or tap here to enter text. |                          |
|               | Apparent Vmax            | Apparent Km              |
| Inhibitor 3   | Click or tap here to enter text. | Click or tap here to enter text. |
|               | Type of inhibition       |                          |
|               | Click or tap here to enter text. |                          |
Transcribed Image Text:**Module 5 Week 1 Assignment: Enzymes** This is a group assignment. You must work with a partner. Please be sure to have joined a group in Canvas and then upload one file for the group. The following kinetic data were obtained for an enzyme in the absence of inhibitor (1) or in the presence of two different inhibitors (2 and 3). The enzyme concentration was the same in all of the experiments. Graph these data as a Lineweaver-Burke plot. | [S] mM | v (umol/ml * sec) | |--------|--------------------| | | Enzyme (1) | Inhibitor (2) | Inhibitor (3) | | 1 | 12 | 4.3 | 5.5 | | 2 | 20 | 8 | 9 | | 4 | 29 | 14 | 13 | | 8 | 35 | 21 | 16 | | 12 | 40 | 26 | 18 | a. Paste a photo of your Lineweaver-Burke plot here. Plot all three lines on one graph. b. Using the data, fill in the following table: | | Vmax | Km | |---------------|--------------------------|--------------------------| | Enzyme | Click or tap here to enter text. | Click or tap here to enter text. | | | Apparent Vmax | Apparent Km | | Inhibitor 2 | Click or tap here to enter text. | Click or tap here to enter text. | | | Type of inhibition | | | | Click or tap here to enter text. | | | | Apparent Vmax | Apparent Km | | Inhibitor 3 | Click or tap here to enter text. | Click or tap here to enter text. | | | Type of inhibition | | | | Click or tap here to enter text. | |
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON