What ion or ions are required for PYC activity? What ion or ions inhibit PYC activity? Remember, Mg2+ is the control substance, and not expected to have any effect on the enzyme’s activity. The catalytic properties of the PYC enzyme were assessed following purification. Theactivity of the enzyme was assayed in the presence of ATP, pyruvate, bicarbonate andMg²+ ions (these latter ions were the control substance). In addition, the dependence ofthe enzyme on these various metabolites was tested by replacing them with similarcompounds. The activity of the enzyme in the presence of these various molecules isshown in the table.EffectorControlAvidinAlternate nucleotides replacing ATPAMPADPCTPGTPITPUTPNucleotides in addition to ATPAMP:t.ts otoplADPsaslyxochsoCTPmubiriqoriolusGTPITPUTP oid levurg ITA 10 soTricarboxylic acid cycle-related compoundsAcetyl-CoAAspartateGlutamatea-ketoglutarateDivalent cations replacing Mg²+Mn²2+2+Activity (% of control)1000alog gni0 You aob-non bas gainoitibbs 01.IS stugil msuld logosidilsos digwordt-om a besno OY9 ort104731069480105849195731746qodi 30 zoitisqoidnoitibeCoZn²+ shaving modto of bensquito esw nog diom oli meil Y1 to

The reaction catalyzed by pyruvate carboxylase (PYC) is given below. Pyruvate + HCO3- + ATP →Mg2+ ,…

Answered: What ion or ions are required for PYC…

What ion or ions are required for PYC activity? What ion or ions inhibit PYC activity? Remember, Mg2+ is the control substance, and not expected to have any effect on the enzyme’s activity.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

What ion or ions are required for PYC activity? What ion or ions inhibit PYC activity? Remember, Mg2+ is the control substance, and not expected to have any effect on the enzyme’s activity.

The catalytic properties of the PYC enzyme were assessed following purification. The
activity of the enzyme was assayed in the presence of ATP, pyruvate, bicarbonate and
Mg²+ ions (these latter ions were the control substance). In addition, the dependence of
the enzyme on these various metabolites was tested by replacing them with similar
compounds. The activity of the enzyme in the presence of these various molecules is
shown in the table.
Effector
Control
Avidin
Alternate nucleotides replacing ATP
AMP
ADP
CTP
GTP
ITP
UTP
Nucleotides in addition to ATP
AMP
:t.ts otopl
ADP
saslyxochso
CTP
mubiriqoriolus
GTP
ITP
UTP oid levurg ITA 10 so
Tricarboxylic acid cycle-related compounds
Acetyl-CoA
Aspartate
Glutamate
a-ketoglutarate
Divalent cations replacing Mg²+
Mn²
2+
2+
Activity (% of control)
100
0
alog gni
0 You a
ob-non bas gai
noitibbs 01.IS stugil m
suld logosidilsos digwordt
-om a besno OY9 ort
104
73
106
94
80
105
84
91
95
73
17
46
qodi 30 zoitisqoid
noitibe
Co
Zn²+ shaving modto of bensquito esw nog diom oli meil Y1 to

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