What ion or ions are required for PYC activity? What ion or ions inhibit PYC activity? Remember, Mg2+ is the control substance, and not expected to have any effect on the enzyme’s activity.

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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What ion or ions are required for PYC activity? What ion or ions inhibit PYC activity? Remember, Mg2+ is the control substance, and not expected to have any effect on the enzyme’s activity.

The catalytic properties of the PYC enzyme were assessed following purification. The
activity of the enzyme was assayed in the presence of ATP, pyruvate, bicarbonate and
Mg²+ ions (these latter ions were the control substance). In addition, the dependence of
the enzyme on these various metabolites was tested by replacing them with similar
compounds. The activity of the enzyme in the presence of these various molecules is
shown in the table.
Effector
Control
Avidin
Alternate nucleotides replacing ATP
AMP
ADP
CTP
GTP
ITP
UTP
Nucleotides in addition to ATP
AMP
:t.ts otopl
ADP
saslyxochso
CTP
mubiriqoriolus
GTP
ITP
UTP oid levurg ITA 10 so
Tricarboxylic acid cycle-related compounds
Acetyl-CoA
Aspartate
Glutamate
a-ketoglutarate
Divalent cations replacing Mg²+
Mn²
2+
2+
Activity (% of control)
100
0
alog gni
0 You a
ob-non bas gai
noitibbs 01.IS stugil m
suld logosidilsos digwordt
-om a besno OY9 ort
104
73
106
94
80
105
84
91
95
73
17
46
qodi 30 zoitisqoid
noitibe
Co
Zn²+ shaving modto of bensquito esw nog diom oli meil Y1 to
Transcribed Image Text:The catalytic properties of the PYC enzyme were assessed following purification. The activity of the enzyme was assayed in the presence of ATP, pyruvate, bicarbonate and Mg²+ ions (these latter ions were the control substance). In addition, the dependence of the enzyme on these various metabolites was tested by replacing them with similar compounds. The activity of the enzyme in the presence of these various molecules is shown in the table. Effector Control Avidin Alternate nucleotides replacing ATP AMP ADP CTP GTP ITP UTP Nucleotides in addition to ATP AMP :t.ts otopl ADP saslyxochso CTP mubiriqoriolus GTP ITP UTP oid levurg ITA 10 so Tricarboxylic acid cycle-related compounds Acetyl-CoA Aspartate Glutamate a-ketoglutarate Divalent cations replacing Mg²+ Mn² 2+ 2+ Activity (% of control) 100 0 alog gni 0 You a ob-non bas gai noitibbs 01.IS stugil m suld logosidilsos digwordt -om a besno OY9 ort 104 73 106 94 80 105 84 91 95 73 17 46 qodi 30 zoitisqoid noitibe Co Zn²+ shaving modto of bensquito esw nog diom oli meil Y1 to
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