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Kymograph Of A Frog Lab Report

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A live specimens of (Xenopus leavis ) were removed from a bucket, tweezers were used to open its mouth and bone crushing scissors were placed between its mouth and behind its eye. The top portion of the head was then cut off, killing the frog instantly. A dissecting needle was gently shoved in the spinal cord and it was moved around in order to kill the nerve in the spinal cord. The frog was placed onto its back and dissecting scissors were used to cut the skin across the abdomen. The frog was pinned down as one member then pulled the skin off the frog down towards its lower limbs. This process was seen as “pulling off the pants”. Ringer solution was added to the tissues using a clean pipette. One leg was chosen and dissecting scissors were …show more content…

Paper was wrapped around the drum of the Kymograph using tape. Water was placed in the outer flask of the enterograph (Figure 1) with a beaker. The inner flask was filled with Ringer solution and the machine was then plugged in and the temperature was set to full heat. Before adding the Ringer solution to the inner flask, a clamp was placed on the end of a tube leading to the inner flask with Ringer in order to prevent leakage. One end of the muscle was tied tightly with string, as well as the other end. The length of the string had to be short enough in order to maintain that tension between the muscle and the pen of the enterograph. The string from one end of the muscle was then attached to a small metal hook on a metal rod that was then placed and adjusted into the flask with the Ringer solution, together with the muscle. The other end was then tied onto the end of thin metal rod that could move up and down. A small weight was placed towards the end of the metal rod to counter balance the weight of the kymograph pen that was placed on the front end of the metal rod. There had to be tension on the string so that the muscular movements (if any) could be observed. The gastrocnemius muscle had to be completely submerged in the Ringer solution. A thermometer was placed into the water filled outer flask. Just before the temperature reached 38°C the pen on the enterograph was moved closer to the rotating drum of the kymograph (Figure 2). At 38°C the pen recorded the muscular contraction in the rotating drum. This was done for 47°C and

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