A Experiment On Hydrogen Peroxide

Weight 30 dry pre-82 pennies which get 89.77g, using 30ml initial volume measuring the volume of 30 pennies, record the data 10.0ml. Using equation Density= Mass/Volume, get the density of the pre-82 pennies is 8.98g/ml. Then calculate the error%=0.10%, and the deviation%=1.29%.

Restate your hypothesis: If the mass of liver increases when put in to the same amount of Hydrogen Peroxide, then more oxygen will be produced in the same amount of time. The amount of catalase that reacts with the substrate (Hydrogen Peroxide) increases when we increase the mass of liver inside. As a result, more oxygen will be produced when the mass of liver is increased in the same amount of time. - Does your data/graph support your hypothesis?

The more acidic a substance is the less oxygen it will produce when going through a chemical reaction. During the Lab “How Do Changes in pH Levels Affect Enzymes Activity”, the researcher conducted an experiment to test the effects that an acidic, neutral, and a base substance will have when combine it with hydrogen peroxide. The data table shows that HCL (acidic substance) barley produced any oxygen at all when it was combining with Hydrogen Peroxide. The pH level for HCL was 2.5; this level indicates that the substance was very acidic. When the H2O and NaOH were tested they produced more bubbles than HCL. NaoH produced a little more bubbles than HCL. The pH that NaoH produced was a 9, which is a base. H2O produced more bubbles than both substances;

Andra Larade Bio 30 B7 11/20/15 Liver Enzyme Lab The Effect of pH and Temperature on the Efficiency of Catalase Abstract: In order to determine how pH and temperature affect the liver enzyme catalase, an experiment was conducted. Catalase breaks apart hydrogen peroxide producing water and oxygen.

What is the effect of the concentration of a hydrogen peroxide solution on catalase activity?

The purpose of this laboratory experiment is to explore the effects of pH has on a reaction rate. The reaction studied was the breakdown of hydrogen peroxide catalyzed by enzyme peroxidase. Peroxidase is a large protein containing heme co factors in its active site. Four trials were ran at pH levels of 3, 5, 7, and 9. I hypothesized that the reaction would run very quickly at a pH 7, since that is the normal condition of cells where peroxidase is found.

The topic of this lab is on biochemistry.This experiment was conducted to show how cells prevent the build of hydrogen peroxide in tissues. My group consisted of Lekha, Ruth, and Jason. There were used two different concentrations of hydrogen peroxide through this experiment , 1.5% and 3%. By testing two different types it is easier to understand how the H2O2 and catalase react with one another. To do this both the yeast, which was our catalase, and H2O2 were mixed together in a beaker. Each concentration was tested out twice for more accurate results . 1.5% concentrated H2O2 had an average reaction rate of 10.5 seconds while 3% concentrated H2O2 had an average reaction rate of 7.5 seconds. From this experiment we learned that by increasing the concentration of H2O2 and chemically combining it with a catalase it will speed up the reaction. Enzymes speed up chemical reactions . The independent variable in this experiment was the concentration of the H2O2. Some key vocabulary words are Catalase, enzyme, hydrogen peroxide ( H2O2), and concentration.

In this above reaction, oxygen is released and is used for other cellular purposes, but when it occurs in a test tube, similar to this experiment, the oxygen gas bubbles producing a layer of foam on the surface of the peroxide. The amount of foam and the speed it is produced are forms of measuring the catalase activity. In the next experiments, one would determine the degree of catalase action by calculating the thickness of the foam layer. It is hypothesized that when reacting with: potato, apple, steak, or liver, the plants and animal tissues will react differently.

The purpose of this lab was to explore how certain quantities of hydrogen peroxide would affect the amount of oxygen gas produced in the following lab. The hypothesis of the lab in question was that, if the amount of Hydrogen peroxide added to the catalase (liver) solution increased, then the amount of oxygen gas produced would be much greater. This is because the greater amount of hydrogen peroxide in a catalase solution will result in there being more solution to deform into oxygen. Evidently after this experiment the hypothesis stated has been proven correct, as the quantity of hydrogen peroxide increased the amount of oxygen gas produced also increased. This can proven as at 10 ml there was 45.2cm^3 and at

Each cube was placed in separate Hydrogen Peroxide Mixtures. The resulting oxygen produced from the decomposition was recorded in ml, from the downward displacement of water (see fig 1.1). Results were taken every minute for 20 minutes.

Conclusion How Does increasing the concentration of Hydrogen Peroxide affect the amount of oxygen

Observing how the enzyme catalase found in chicken and beef livers breaks down hydrogen peroxide at varying pH levels and temperatures.

There were three test tubes in which the experiment was held. A relatively equal sized portion of raw potato (this contained the enzyme [a biological catalyst] hydrogen peroxidase) was placed in each tube. Then, enough water to cover the potato was added. Proceeding this, each of the test tubes were assigned a temperature; cold, room temperature or warm (this was written on the tag so that they were not confused). The test tube destinated ‘cold’ was placed in a ice bath for five minutes. At the same time, the ‘hot’ test tube was placed in a hot water bath for five minutes. Meanwhile, the room temperature test tube sat at room temperature for five minutes. When the five minutes were over, the test tubes were returned to the rack (so that they were able to be observed). Then, the test tubes were allowed to sit at room temperature for five more minutes. Once that period of time was over, 2 ml of hydrogen peroxide (the substrate) was added to each tube.

The research and observations of this lab primarily focused on the enzyme activity of the enzyme Peroxidase. Peroxidase is a large protein and is composed of more than three hundred amino acids. The enzyme was selected as it is easy to experiment with and effectively showcases the effects of varying independent variables, such as pH and temperature. Peroxidase catalyzes the decomposition reaction of the chemical Hydrogen Peroxide ( H2 O2 ) into water and an electron donating molecule, which stands for R in the written chemical equation. ( The equation is displayed below:

Catalase breakdown hydrogen peroxide into water and oxygen. This experiment was to test the reaction on catalytic activity