EEI Daniel Blinks YR. 11 Background – Catalase are an enzyme that catalyses the reduction of

Peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide, H2O2, into O2 gas and water. We examined the different pH environments that can affect the enzyme activity during the breakdown of H2O2. In order to do this, we added different levels of pH, low, medium, and high, into different test tubes with the enzyme and H2O2, and we then inverted the tube. The amount of O2 gas produced was then measured and recorded. The result was that the higher pH produced more gas, followed by medium pH, then low pH. The enzymes were more active in the pH of about 10. It increased

Hydrate the yeast packets in a beaker with 400 mL of distilled water at a 10% concentration. In a 50 mL

The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes, temperature, and PH level).

The hypothesis is that catalase activity will increase exponentially with higher concentrations of hydrogen peroxide until all catalase active sites are filled, in which case the

The topic of this lab is on biochemistry.This experiment was conducted to show how cells prevent the build of hydrogen peroxide in tissues. My group consisted of Lekha, Ruth, and Jason. There were used two different concentrations of hydrogen peroxide through this experiment , 1.5% and 3%. By testing two different types it is easier to understand how the H2O2 and catalase react with one another. To do this both the yeast, which was our catalase, and H2O2 were mixed together in a beaker. Each concentration was tested out twice for more accurate results . 1.5% concentrated H2O2 had an average reaction rate of 10.5 seconds while 3% concentrated H2O2 had an average reaction rate of 7.5 seconds. From this experiment we learned that by increasing the concentration of H2O2 and chemically combining it with a catalase it will speed up the reaction. Enzymes speed up chemical reactions . The independent variable in this experiment was the concentration of the H2O2. Some key vocabulary words are Catalase, enzyme, hydrogen peroxide ( H2O2), and concentration.

Cell respiration is the most prevalent and efficient catabolic pathway. It require transfusions of energy from outside sources to perform their many tasks. The the objective of this experiment was to measure the concentration of oxygen gas and carbon dioxide gas. Also to determine whether germinating peas respire. The hypothesis would be that germinating

To performed the experiment, a volumeter was set up to measure the net oxygen production under white light, then a second step was followed to measure oxygen consumption under dark conditions (oxygen production only happens in the presence of light and oxygen consumption in the presence of dark light) and finally, a third step consisted of recording the measure of the net oxygen production under the presence of green light.

This experiment is designed to analyze how the enzyme catalase activity is affected by the pH levels. The experiment has also been designed to outline all of the directions and the ways by which the observation can be made clearly and accurately. Yeast, will be used as the enzyme and hydrogen peroxide will be used as a substrate. This experiment will be used to determine the effects of the concentration of the hydrogen peroxide versus the rate of reaction of the enzyme catalase.

If the temperature is too hot or too cold, then the reactivity and reaction rate of which the enzyme catalase breaks down hydrogen peroxide will decrease.

Therefore, following the removal of these points, the variance (s2) and the standard deviation (s) of the data that these points belong to, decrease greatly. 5.1: QUALITATIVE OBSERVATIONS The colour of the solution remained constant; a very beige colour. The 100.0 ± 0.5 cm3 graduated cylinder indicated 5.0 ± 0.5 cm3 of oxygen gas within 25.00 ± 0.01 s, whereas more than 25.00 ± 0.01 s was required to produce the remaining 5 ± 0.5 cm3 of O2 (g).

There were three test tubes in which the experiment was held. A relatively equal sized portion of raw potato (this contained the enzyme [a biological catalyst] hydrogen peroxidase) was placed in each tube. Then, enough water to cover the potato was added. Proceeding this, each of the test tubes were assigned a temperature; cold, room temperature or warm (this was written on the tag so that they were not confused). The test tube destinated ‘cold’ was placed in a ice bath for five minutes. At the same time, the ‘hot’ test tube was placed in a hot water bath for five minutes. Meanwhile, the room temperature test tube sat at room temperature for five minutes. When the five minutes were over, the test tubes were returned to the rack (so that they were able to be observed). Then, the test tubes were allowed to sit at room temperature for five more minutes. Once that period of time was over, 2 ml of hydrogen peroxide (the substrate) was added to each tube.

Sulfuric acid - was used to stop the reaction with catalase and hydrogen peroxide. It denatured the enzyme (catalase) and halted the reaction so the amount of hydrogen peroxide decomposed could be measured.

Next, fifteen drops of potato juice were added to each test tube. Simultaneously, ten drops of Hydrogen Peroxide (H2O2) were added in all test tubes at the same time. All test tubes were gently shaken to mix the solutions and put to rest. After three minutes, the height of the bubbles of the three test tubes were measured at the same time. Then, the test tubes were exposed to different temperatures.

The aim of my investigation is to see how pH affects the activity of potato tissue catalase, during the decomposition of hydrogen peroxide to produce water and oxygen.

According to the results of the lab, it appears that hydrogen peroxide can be broken down by catalysts other than enzymes; such as magnesium dioxide. In the lab, hydrogen peroxide barely reacted with the sand (thus indicating its lack or absence of catalase). However, when the hydrogen peroxide was paired with magnesium dioxide, a significantly greater reaction occurred. Therefore indicating that hydrogen peroxide can in fact be broken down by (inorganic and organic) catalysts other than enzymes.