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How can measuring the activity of the acid phosphatase of the prostrate gland in human blood serum by using tartrate ion?
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- Can you help me to find procedure for measuring the activity of the acid phosphatase of the prostrate gland in human blood serum ?What would happen to the patient if the serum cholinesterase activity were only 10 units of activity per liter rather than the normal activity of about 80 units?how to measure total activity of the acid phosphatase enzyme by using Tartrate in blood serum?
- Why valinomycin is highly selective for K+ relative to Na+? (Subject: Biomembrane)One of the important uses of the Nernst equation is in describing the flow of ions across plasma membranes. Ions move under the influence of two forces: the concentration gradient (given in electrical units by the Nernst equation) and the electrical gradient (given by the membrane voltage). This is summarized by Ohms law: Ix=Gx(VmEx) which describes the movement of ion x across the membrane. I is the current in amperes (A); G is the conductance, a measure of the permeability of x, in Siemens (S), which is I/V;Vm is the membrane voltage; and Ex is the equilibrium potential of ion x. Not only does this equation tell how large the current is, but it also tells what direction the current is flowing. By convention, a negative value of the current represents either a positive ion entering the cell or a negative ion leaving the cell. The opposite is true of a positive value of the current. a. Using the following information, calculate the magnitude of Na [ Na+ ]0=145mM,[ Na+ ]i=15mM,Gna+=1nS,Vm=70mV b. Is Na+ entering or leaving the cell? c. Is Na+ moving with or against the concentration gradient? Is it moving with or against the electrical gradient?Using the Nernst equation, calculate the equilibrium potential for Ca2 and for C1 from the following sets of data: a. Given [ Ca2+ ]0=1mM,[ Ca2+ ]i=100nM, find Eca2+ b. Given [ Cl- ]0=110mM,[ Cl- ]i=100mM, find Ecl
- Intracellular concentrations in resting muscle are as follows: fructose6-phosphate, 1.0 mM; fructose-1,6-bisphosphate, 10 mM; AMP, 0.1 mM;ADP, 0.5 mM; ATP, 5 mM; and Pi, 10 mM. Is the phosphofructokinasereaction in muscle more or less exergonic than under standard conditions?By how much?Explain the mechanism how furosemide affects kidney tubules. How does this affect K+ balance? How different is the mechanism of thiazide affect urine production? Describe the process of acid-base regulation in human body.How does moderate to severe COPD (chronic obstructive pulmonary disease) often lead to chronic respiratory acidosis? How might the kidneys compensate for this i.e explain at least 2 mechanisms which the kidney may use to do so - be sure to include hormones, cells involved. If the H+/K+ ATPase is brought into service, how would the plasma K+ concentration be affected? Lastly, how would activation of H+/K+ ATPase counteract or synergize with the actions of aldosterone?
- Suppose that a macro molecule(M) inside a dialysis bag with a net charge of +24 at a concentration of 1.0mM is dialyzed against a NaCl concentration of 0.54M what are the concentration ratio(r) of both Na+ and Cl- for inside to outside the bage?If lactic acid is the buildup product of strenuous muscle activity, why is sodium lactate often given to hospital patients intravenously?Hemoglobin molecules exposed to high levels of glucose areconverted to glycated products. The most common, referred to as hemoglobin A1C (HbA1C), contains a b-chain glycatedadduct. Because red blood cells last about 3 months, HbA1Cconcentration is a useful measure of a patient’s blood sugarcontrol. In general terms, describe why and how HbA1Cforms.