A person did estimation of blood glucose level by glucose oxidase method. But he forgot to add ortho - toluidine reagent during glucose oxidase reagent preparation. what is the observation of the result?
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A person did estimation of blood glucose level by glucose oxidase method. But he forgot to
add ortho - toluidine reagent during glucose oxidase reagent preparation.
what is the observation of the result?
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- Urine samples for each patient were tested for ketone bodies, and a Benedict's reagent and glucose oxidase reaction was also completed. The results are given in the table below, with - indicating a negative result and +, ++ and +++ indicating a positive result of different intensity. Patient A Patient B Patient C Patient D Patient E Ketone Bodies +++ Benedict's Reagent +++ - +++ - +++ Glucose Oxidase +++ +++ + 4. For each individual patient A-E, using the WHO guidelines for the diagnosis of diabetes, write a short paragraph to explain: (a) what you can deduce from the fasting blood glucose and the 2 hour sample of that patientIf a sample is composed of a mixture of maltose, maltodextrin, and glucose, which of the following is expected? Adding alkaline CuSO4 to the solution will turn the solution into orange with some dark red precipitate. Performing Molisch Test on the sample will give a negative result. Reaction of the sample with phenylhydrazine will give a solution with yellow precipitate. Furthermore, inspection of the precipitate under the microscope will show cotton ball shaped crystals. . The Osazone Test on the sample will give a yellow solution with no visible precipitate.Urine samples for each patient were tested for ketone bodies, and a Benedict's reagent and glucose oxidase reaction was also completed. The results are given in the table below, with indicating a negative result and +, ++ and +++ indicating a positive result of different intensity. Patient A Patient B Patient C Patient D Patient E Ketone Bodies - +++ Benedict's Reagent +++ +++ +++ Glucose Oxidase +++ +++ + 4. For each individual patient A-E, using the WHO guidelines for the diagnosis of diabetes, write a short paragraph to explain: (b) whether this deduction is confirmed by the detection of ketone bodies and sugar in the urine samples from that patient ||
- Explain the role of L-tartrate in the determination of acid phosphatase . Differentiate thymolphthalein monophosphate method from alpha naphthyl phosphate method of prostatic acid phosphate determination. Why is acid phosphatase determination useful in establishing recent sexual intrusion as in the case of alleged rape? EXPLAIN 3-5 SENTENCESSerum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If serum stays at low temperature (40) for several hours fatty layer appears on its surface. What are the possible causes of these symptoms? To explain this, answer the questions and do the following tasks: a) what compounds of serum must be tested for that patient in biochemical lab? b) write the reaction which does not occur properly in patient’s blood; c) write down the schemes, explaining how the products of the previous reaction are used in adipose tissue and heart in healthy person 2 hours after a meal.In the estimation of glucose with benedict's reagent, what is resposnible for the white precipitate observed? Indicated the reaction responsible for this.
- The following laboratory test results were obtained in a patient with severe jaundice, right upper quadrant abdominal pain, fever, and chills. Serum alkaline phosphatase Four times normalSerum cholesterol IncreasedAST (SGOT) Normal or slightly increased5’-Nucleotidase IncreasedTotal serum bilirubin 25 mg/dLConjugated bilirubin 19 mg/dLProthrombin time Prolonged but improves with a vitamin K injection A) What is the most likely cause of jaundice in the patient? Rationalize your answers.Describe the two phases of the the HMP shunt. Enumerate the reactions in each phase and the corresponding enzyme of each reation. Explain how the HMP shunt is regulated?Absorbance is directly proportional to glucose concentration for both routine clinical glucose spectrophotometry methods: glucose oxidase method and the hexokinase method. For each one, name the final product measured in each test reaction, which is proportional to the sample glucose concentration. A.) glucose oxidase: B.) hexokinase:
- Give the role of starch solution, phosphate buffer, and NaCI solution in the amylase test. What are some of the possible outcomes of the test?Make an experimental procedure for the synthesis of ascorbic acid from glucose:Tyrosinase enzyme activity is assayed by monitoring the oxidation of 3, 4-dihydroxyphenylalanine (dopa) to the red-colored dopachrome. Calculate the tyrosinase activity (U/mL) by using the experimental data given below