Explain the role of copper sulfate, CuSO4 as a component of the Benedict’s reagent for the Estimation of Glucose
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Explain the role of copper sulfate, CuSO4 as a component of the Benedict’s reagent for the Estimation of Glucose
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- Explain the trisodium citrate, sodium carbonate, potassium thiocyanate, potassium hexacyanoferrate, and copper sulfate components of Benedict’s reagent used for Estimation of GlucoseIn the estimation of glucose with benedict's reagent, what is resposnible for the white precipitate observed? Indicated the reaction responsible for this.In glucose estimation using Benedict's Reagent, what is/are the chemical reaction/s that resulted in the white precipitate observed.
- Why is Molisch’s test used for the determination of presence of pentose in thehydrolysate? What other test could be used for this?Discuss briefly the principle involved in the estimation of blood glucose by the glucose oxidase method.Briefly describe the function of uridine triphosphate (UTP) in carbohydratemetabolism.
- Describe the two phases of the the HMP shunt. Enumerate the reactions in each phase and the corresponding enzyme of each reation. Explain how the HMP shunt is regulated?Describe the process of brewing beer by ethanol fermentation of the carbohydrates in cereal grains such as barley, carried out by yeast enzymes. Discuss the biochemical basis of the processes involved in malting, preparing the wort, fermentation by yeast, and in the final step of brewing, controlling the amount of foam on the beerThe lipase substrate emulsion contains 0.500 mg of olive oil per 3 mL Also, the molar mass of the olive oil is 885 g/mol. If all of the olive oil in a 3 mL reaction is "digested" by pancreatic lipase in a reaction, how many molecules of olive oil have been degraded?
- Examine the ActiveModel for alcohol dehydrogenase and describe the structure and function of the catalytic zinc center.Urine samples for each patient were tested for ketone bodies, and a Benedict's reagent and glucose oxidase reaction was also completed. The results are given in the table below, with - indicating a negative result and +, ++ and +++ indicating a positive result of different intensity. Patient A Patient B Patient C Patient D Patient E Ketone Bodies +++ Benedict's Reagent +++ - +++ - +++ Glucose Oxidase +++ +++ + 4. For each individual patient A-E, using the WHO guidelines for the diagnosis of diabetes, write a short paragraph to explain: (a) what you can deduce from the fasting blood glucose and the 2 hour sample of that patienti) Re-arrange the Michaelis Menten equation so it involves the ratio [S]. Show all steps beginning Km noting any assumptions or required conditions. Km ii) Calculate the ratio [lo for the case when the rate of product formation is 68% of Vmax and the substrate is in great excess. d[P] dt : k₂ with = [E],[S] Km+[S]' [S]o Km iii) Explain, in a few sentences, why the ratio determines the ratio V Vmax V Vmax Begin by explaining the meaning of stating simply "it's the ratio...." is not sufficient. Include in your explanation the factors that effect v and Vmax. Consider what factors make v different from or equal to Vmax. Consider what Km represents concerning processes involving ES. " iv) Calculate KM at 310K at given the following rate constant information: k₁ = 17 s-¹M-1 at 300K with A = 7300 s-¹M-1 K-1₁ 6 s¹ at 300K with A = 14500 s -1 k₂ = 31 s¹ at 300K with A = 600 s-¹