Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 17, Problem 12TYK
Discuss Concepts As a control for their experiments with bacterial recombination, Lederberg and Tatum placed cells of either “parental” strain 1 or 2 on the surface of a minimal medium. If you set up this control and a few scattered colonies showed up, what might you propose as an explanation? How could you test your explanation?
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In the experiment performed by Messelson and Stahl, bacterial cells grown in 15N-containing growth medium for many generations were then allowed to grow in 14N-containing medium.
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one band of low density (I.e. light band), one band of intermediate density, and one band of high density (i.e. heavy band).
In the experiment performed by Messelson and Stahl, bacterial cells grown in 15N-containing growth medium for many generations were then
allowed to grow in 14N-containing medium. Suppose they would have conducted the experiment the other way around: that is, to first grow bacterial
cells in 14N-containing medium for many generations and then switching them to a 15N-containing growth medium. After two generations of growth
in 15N media the results of a CsCl density gradient centrifugation of the DNA would be expected to show
one band of intermediate-density and one band of low-density (i.e. light band)
one band of low density (i.e. light band), one band of intermediate density, and one band of high density (i.e. heavy band).
one band of intermediate density and one band of high-density (i.e. heavy band)
one band on intermediate density
one band of low density (i.e. light band) and one band of high density (i.e. heavy band).
O O
In the Hershey Chase experiment, why was the localization of radio-labeled phage of macromolecules in the cytoplasm the reason that the genetic material identity was proved.
Chapter 17 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 17.1 - What are the properties of F+, F-, and Hfr cells...Ch. 17.1 - Prob. 2SBCh. 17.2 - Prob. 1SBCh. 17.2 - How does viral infection of an animal cell and a...Ch. 17.2 - Prob. 3SBCh. 17.3 - Prob. 1SBCh. 17 - Prob. 1TYKCh. 17 - Prob. 2TYKCh. 17 - Which of the following is not correct for...Ch. 17 - Prob. 4TYK
Ch. 17 - Prob. 5TYKCh. 17 - Prob. 6TYKCh. 17 - When a virus enters the lysogenic stage: a. the...Ch. 17 - An infectious material is isolated from a nerve...Ch. 17 - Prob. 9TYKCh. 17 - Prob. 10TYKCh. 17 - Prob. 11TYKCh. 17 - Discuss Concepts As a control for their...Ch. 17 - Prob. 13TYKCh. 17 - Prob. 14TYKCh. 17 - Prob. 15TYKCh. 17 - Prob. 1ITD
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- By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:What information do you know based on the question and your understanding of the topic?arrow_forwardBy conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Analyze data. Compare and contrast. Make a drawing.arrow_forwardWhen plasmids are isolated from bacterial cells, they may existin a number of forms.a. List the different forms that may be found.b. Which do you think would migrate the fastest and farthest in anelectrophoresis experiment and why?arrow_forward
- You co-culture the following bacterial strains: an Hfr prototroph and an F- auxotroph for the genes mal, met, mtl, and xyl. You interrupt conjugation at various time points and place the mixtures on media plates lacking each of the nutrients. Based on the results shown in the image, what is the order of these four genes along the bacterial chromosome? Please explain!arrow_forwardLinkage maps in an Hfr bacterial strain are calculated in units of minutes (the number of minutes between genes indicates the length of time that it takes for the second gene to follow the first in conjugation). In making such maps, microbial geneticists assume that the bacterial chromosome is transferred from Hfr to F − at a constant rate. Thus, two genes separated by 10 minutes near the origin end are assumed to be the same physical distance apart as two genes separated by 10 minutes near the F −attachment end. Suggest a critical experiment to test the validity of this assumption.arrow_forwardIn the experiment below, flasks of human fibroblasts are either transfected with a control gene or the v-src gene. After 48 hrs the flasks (Top row) as well as microscope images of cells on surface of flask (bottom row) from the two different experiments are depicted. Which Flask (A or B) is from the v-src transfection? Justify in 3-4 sentences why you drew this conclusion. Flask A Flask B iumarrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license