In the experiment below, flasks of human fibroblasts are either transfected with a control gene or the v-src gene. After 48 hrs the flasks (Top row) as well as microscope images of cells on surface of flask (bottom row) from the two different experiments are depicted. Which Flask (A or B) is from the v-src transfection? Justify in 3-4 sentences why you drew this conclusion. Flask A Flask B ium

In the experiment below, flasks of human fibroblasts are either transfected with a control gene or the v-src gene. After 48 hrs the flasks (Top row) as well as microscope images of cells on surface of flask (bottom row) from the two different experiments are depicted. Which Flask (A or B) is from the v-src transfection? Justify in 3-4 sentences why you drew this conclusion. Flask A Flask B ium

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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In the "Bacterial Transformation" experiment, why were there no fluorescence bacteria present in the other plates even though these were inserted by the +pGLO plasmid? (Note: Discuss the importance on the addition of ampicillin and arabinose to the medium of the genetically engineered bacteria). Limit your answer to 1-3 sentences only.
You are counting plaques on your plaque assay plates made from serial dilutions of your high titer lysate. Your 10-5 plate has 615 plaques although some are butting up against each other so it is difficult to get an accurate count. Your 10-6 plate has 42 plaques, and your 10-7 plate has only 1 plaque. Which plate would probably yield the most accurate titer calculation of your phage and why is it more trustworthy than the others?
Ms. Carla was required to clone three different genes (Gcne 1, Gene 2 and Gene 3) into PUC18. After generating three recombinant plasmids, she transformed them into the Escherichiu coli host cells and plated them on the LB agar plates containing X-gal. After a 16-hour incubation peried, she obtained different outcomes for all of her three genes of interest. Gene 1 plate: No colonies ut all Gene 2 plate: All blue colonies Gene 3 plate: All white colonies Explain the results obtained for cach plate with clear justification.
Eight mutant bacteriophage strains cannot lyse a certain type of bacteria that can be lysed by wild-type bacteriaphages. The mutant strains were allawed to infect the bacteria in a complementation test. A "+ indicates that lysis occurred with coinfection.Aindicates that lysis did not occur. GKWTMAQC G -++|+ ++l+ - K. - + - ++ +| + -+| - |-++ + + + M - + + + A cistron is defined by no complementation in the Ciş : configuration. Haw many genes are controlling lysis in this bacteriophage? (Use a number not a word in the space) 3 Which of the following strains below are defective in the same gene as strain Q? Answer yes if the strain is defective or no if it is not. Strain Defective A N/A
Considering counting rules, calculate the initial titre of the sample viral stock if the following plaques were counted in a plaque assay. Show clear sample calculations. Table 1: Plaque assay counts for lambda phage stock titre enumeration of average concentration (PFU/mL) Total dilution of viral stock 1/13500000 1/135000000 1/135000000 plated Volume diluted viral stock 0.1 0.1 0.1 plated (mL) PFU replicate 1 TNTC 275 25 Calculated concentration (PFU/mL) replicate 1 PFU replicate 2 TNTC 239 23 Calculated concentration (PFU/mL) replicate 2 Average concentration (PFU/mL)
Shown below are the complementation test results involving 4 independently isolated lethal mutants in a bacteriophage. Complementation was assayed by simultaneouly infecting bacteria with two phage strains, each with a different mutation, neither of which could alone lyse the cells. In the table below, a "+" indicates the strains complemented each other and therefore lysed open the bacteria. A "0" indicates no complementation and therefore no cell lysis occurred. Test pair Results 1___2___3___4 1,2 + 1 0 + + 0 1,3 + 2 0 + + 1,4 0 3 0 + 2,3 + 4 0 2,4 + 3,4 + How many genes are there? a. 3 b.1 c. 2 d. 4
Shown below are the complementation test results involving 4 independently isolated lethal mutants in a bacteriophage. Complementation was assayed by simultaneouly infecting bacteria with two phage strains, each with a different mutation, neither of which could alone lyse the cells. In the table below, a "+" indicates the strains complemented each other and therefore lysed open the bacteria. A "0" indicates no complementation and therefore no cell lysis occurred. Test pair Results 1___2___3___4 1,2 + 1 0 + + 0 1,3 + 2 0 + + 1,4 0 3 0 + 2,3 + 4 0 2,4 + 3,4 + Which mutants are in the same gene? . a. 2, & 3 b.1, 2, 3 & 4 c.1 & 4 d.1, 2 & 4
ITS DNA was extracted from the same plant but several trials were loaded separately from lanes 2-11. Lane 1 is a 1kbp DNA ladder. No controls were used in this gel matrix. 4 5 6 7 8 9. 10 11 a) Which lanes show a positive result in the amplification? b) Label the fragment sizes of the ladder (Attach the same image).
You infect bacteria with two phages during a cis test. This produces a lysate that contains phage particles. Before you can count outcomes, you need to work out the amount of phage to use in subsequent experiments. You start by making two ten- fold serial dilutions of the lysate and then add 1mL of the final diluted lysate to a plate of permissive strain e.coli. You observe 10 plaques. How many phage per mL were present in the original lysate? a. 10 phage/mL o b. 3,300 phage/mL c. 10,000 phage/mL o d. 1,000 phage/mL e. 33 phage/mL
5 μL of plasmid DNA (pUC 19) was added to 50ul of CaCl2 competent E. coli that was then added 250 μL of SOC medium, 100 μL of this solution was plated onto a TSA + ampicillin plate. Can you please show me how I calculate the total number of successful transformants per mL of competent cells plated. The total number of colnies counted on plate was 70. Thank you
Two auxotrophic triple Escherichia coli strains (A: met- phe- ade- val+ bio+ thr+ and B: met+ phe+ ade+ val- bio- thr-) are mixed in LB liquid medium, diluted and then spread on LB solid rich medium. Six colonies are observed: Then, replicates are performed on 6 different media (minimum medium + glucose + indicated substances). The results are shown below. Determine the genotype of the 6 colonies observed. Which ones are from strain A? From strain B? Which hypotheses can explain these results and which one do you prefer? met phe val bio Abbreviations: met ade val thr phe ade bio thr Met: methionine; Phe: phenylalanine; Ade: adenine; Val: valine; Bio: biotin; Thr: threonine.
E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interference